ArticleScientific reports2019
Deposition of calcium in an in vitro model of human breast tumour calcification reveals functional role for ALP activity, altered expression of osteogenic genes and dysregulation of the TRPM7 ion channel.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- From diagnostic marker-to-therapeutic modality: emerging paradigms in tumor biomineralization.Cancer biology & medicine · 2026Review
- TRP channels and cancer modulation: a voyage beyond metabolic reprogramming, oxidative stress and the advent of nanotechnologies in targeted therapy.Journal of experimental & clinical cancer research : CR · 2025Review
- Moringa oleifera L. Nanosuspension Extract Administration Affects Heat Shock Protein-10 and -70 under Orthodontics Mechanical Force In Vivo.European journal of dentistry · 2025Article
- The Effect of Lanthanum (III) Nitrate on the Osteogenic Differentiation of Mice Bone Marrow Stromal Cells.Biological trace element research · 2024Article
- The Role of TRPM7 in Oncogenesis.International journal of molecular sciences · 2024Review
- PI3K/AKT signaling activates HIF1α to modulate the biological effects of invasive breast cancer with microcalcification.NPJ breast cancer · 2023Article
- Clinical and pathological features analysis of invasive breast cancer with microcalcification.Cancer medicine · 2023Article
- Exploration of utility of combined optical photothermal infrared and Raman imaging for investigating the chemical composition of microcalcifications in breast cancer.Analytical methods : advancing methods and applications · 2023Article
- Surface Modification of Additively Fabricated Titanium-Based Implants by Means of Bioactive Micro-Arc Oxidation Coatings for Bone Replacement.Journal of functional biomaterials · 2022Article
- Osteogenic differentiation of pre-conditioned bone marrow mesenchymal stem cells with Nisin on modified poly-L-lactic-acid nanofibers.Regenerative therapy · 2022Article
- Article
- Review
- Psoralen accelerates osteogenic differentiation of human bone marrow mesenchymal stem cells by activating the TGF-β/Smad3 pathway.Experimental and therapeutic medicine · 2021Article
- A time-course Raman spectroscopic analysis of spontaneous in vitro microcalcifications in a breast cancer cell line.Laboratory investigation; a journal of technical methods and pathology · 2021Article
- Multiple Pathways for Pathological Calcification in the Human Body.Advanced healthcare materials · 2021Review
- Molecular Aspects and Prognostic Significance of Microcalcifications in Human Pathology: A Narrative Review.International journal of molecular sciences · 2020Review
- Molecular Aspects of Thyroid Calcification.International journal of molecular sciences · 2020Review
- Anti-Osteoporotic Effects of Antioxidant Peptides PIISVYWK and FSVVPSPK fromAntioxidants (Basel, Switzerland) · 2020Article
- Review
- Microcalcifications Drive Breast Cancer Occurrence and Development by Macrophage-Mediated Epithelial to Mesenchymal Transition.International journal of molecular sciences · 2019Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microcalcifications are vital mammographic indicators contributing to the early detection of up to 50% of non-palpable tumours and may also be valuable as prognostic markers. However, the precise mechanism by which they form remains incompletely understood. Following development of an in vitro model using human breast cancer cells lines cultured with a combination of mineralisation-promoting reagents, analysis of calcium deposition, alkaline phosphatase (ALP) activity and changes in expression of key genes was used to monitor the calcification process. Two cell lines were identified as successfully mineralising in vitro, MDA-MB-231 and SKBR3. Mineralising cell lines displayed higher levels of ALP activity that was further increased by addition of mineralisation promoting media. qPCR analysis revealed changes in expression of both pro- (RUNX2) and anti- (MGP, ENPP1) mineralisation genes. Mineralisation was suppressed by chelation of intracellular Ca
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