Evidence map›Paper›PMID 30679441›Full record

ArticleScientific reports2019

PD-L1 Expression in Circulating Tumor Cells Increases during Radio(chemo)therapy and Indicates Poor Prognosis in Non-small Cell Lung Cancer.

Yang Wang, Tae Hyun Kim, Shamileh Fouladdel, Zhuo Zhang, Payal Soni, Angel Qin, Lili Zhao, Ebrahim Azizi, Theodore S Lawrence, Nithya Ramnath and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers, 5 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
84citing papers in PubMed, 5 pooled it
8.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

84 citing papers in PubMed, 5 syntheses or guidelines pooled it, 140 citations in OpenAlex.

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  18. Molecular biology of the novel anticancer medications: a focus on kinases inhibitors, biologics and CAR T-cell therapy.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2025
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24 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Yang WangDepartment of Chemical Engineering, University of Michigan, Ann Arbor, MI, USA.
Tae Hyun KimBiointerfaces Institute, University of Michigan, Ann Arbor, MI, USA.
Shamileh FouladdelBiointerfaces Institute, University of Michigan, Ann Arbor, MI, USA.
Zhuo ZhangDepartment of Chemical Engineering, University of Michigan, Ann Arbor, MI, USA.
Payal SoniDepartment of Radiation Oncology, University of Michigan Medical School, Ann Arbor, MI, USA.
Angel QinDepartment of Internal Medicine, University of Michigan Comprehensive Cancer Center, Ann Arbor, MI, USA.
Lili ZhaoDepartment of Biostatistics, University of Michigan, Ann Arbor, MI, USA.
Ebrahim AziziBiointerfaces Institute, University of Michigan, Ann Arbor, MI, USA.
Theodore S LawrenceDepartment of Radiation Oncology, University of Michigan Medical School, Ann Arbor, MI, USA.
Nithya RamnathDepartment of Internal Medicine, University of Michigan Comprehensive Cancer Center, Ann Arbor, MI, USA.
Kyle C CuneoDepartment of Radiation Oncology, University of Michigan Medical School, Ann Arbor, MI, USA. kcuneo@med.umich.edu.
Sunitha NagrathDepartment of Chemical Engineering, University of Michigan, Ann Arbor, MI, USA. snagrath@umich.edu.
University of Michigan–Ann Arbor · USBioSurfaces (United States) · USMichigan Center for Translational Pathology · US

Funding

Label free microfluidic isolation, characterization and ex vivo expansion of CTCsR01CA208335 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI NAGRATH, SUNITHA · 2017 to 2021
$2.8M
High Throughput GO Chip isolation of Lung CTCs for Molecular Diagnosis and Drug TestingR33CA202867 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI NAGRATH, SUNITHA, RAMNATH, NITHYA · 2016 to 2018
$1.0M
NCI NIH HHS R01 CA208335NCI NIH HHS R33 CA202867
6 · The paper itself

Abstract

Preclinical studies demonstrated that radiation up-regulates PD-L1 expression in tumor cells, providing a rationale for combining PD-1/PD-L1 inhibitors with radiation. However this has not been validated in patients with non-small cell lung cancer due to the difficulty to obtain serial biopsies. Measuring PD-L1 expression in circulating tumor cells (CTCs), may allow real-time monitoring of immune activation in tumor. In this study, whole blood from non-metastatic NSCLC patients was collected before, during, and after radiation or chemoradiation using a microfluidic chip. PD-L1 expression in CTCs was assessed by immunofluorescence and qPCR and monitored through the course of treatment. Overall, PD-L1(+) CTCs were detected in 25 out of 38 samples (69.4%) with an average of 4.5 cells/ml. After initiation of radiation therapy, the proportion of PD-L1(+) CTCs increased significantly (median 0.7% vs. 24.7%, P < 0.01), indicating up-regulation of PD-L1 in tumor cells in response to radiation. In addition, patients positive for PD-L1 (≥5% of CTCs positive for PD-L1) at baseline had shorter PFS. Gene expression analysis revealed that higher levels of PD-L1 were associated with poor prognosis. Therefore, CTCs can be used to monitor dynamic changes of PD-L1 during radiation therapy which is potentially prognostic of response to treatment.

Indexed as

B7-H1 AntigenCarcinoma, Non-Small-Cell LungFluorescent Antibody TechniqueGene Expression ProfilingHumansMicrofluidicsNeoplastic Cells, CirculatingPrognosisReal-Time Polymerase Chain ReactionB7-H1 AntigenCD274 protein, human

Identifiers

PMID30679441
PMCPMC6345864
OpenAlexW2920633385

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.