ArticlePlant physiology2019
Modulating Protein Stability to Switch Toxic Protein Function On and Off in Living Cells.
Article in Plant physiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed.
- Manipulation of targeted protein degradation in plant biology.Biochemical Society transactions · 2025Review
- Ubiquitin E3 ligases in the plant Arg/N-degron pathway.The Biochemical journal · 2024Review
- Article
- Using CRL3Plant direct · 2022Article
- Conditional and tissue-specific approaches to dissect essential mechanisms in plant development.Current opinion in plant biology · 2022Review
- Trichome Transcripts as Efficiency Control for Synthetic Biology and Molecular Farming.Methods in molecular biology (Clifton, N.J.) · 2022Article
- New beginnings and new ends: methods for large-scale characterization of protein termini and their use in plant biology.Journal of experimental botany · 2019Review
- Focus Issue Editorial: Synthetic Biology.Plant physiology · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Toxic proteins are prime targets for molecular farming (the generation of pharmacologically active or biotechnologically usable compounds in plants) and are also efficient tools for targeted cell ablation in genetics, developmental biology, and biotechnology. However, achieving conditional activity of cytotoxins and maintaining the toxin-expressing plants as stably transformed lines remain challenging. Here, we produce a switchable version of the highly cytotoxic bacterial RNase barnase by fusing the protein to a portable protein degradation cassette, the low-temperature degron cassette. This method allows conditional genetics based on conditional protein degradation via the N-end rule or N-degron pathway and has been used to vice versa accumulate and/or deplete a diverse variety of highly active, unstable or stable target proteins in different living multicellular organisms and cell systems. Moreover, we expressed the barnase fusion under control of the trichome-specific
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Registered trials
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