Evidence map›Paper›PMID 30678652›Full record

ArticleBMC cancer2019

Cell cycle, energy metabolism and DNA repair pathways in cancer cells are suppressed by Compound Kushen Injection.

Jian Cui, Zhipeng Qu, Yuka Harata-Lee, Thazin Nwe Aung, Hanyuan Shen, Wei Wang, David L Adelson

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cancer, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed, 2 pooled it
3.5field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 2 syntheses or guidelines pooled it, 56 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Jian CuiDepartment of Molecular and Biomedical Science, The University of Adelaide, North Terrace, Adelaide, 5005, South Australia, Australia.
Zhipeng QuDepartment of Molecular and Biomedical Science, The University of Adelaide, North Terrace, Adelaide, 5005, South Australia, Australia.
Yuka Harata-LeeDepartment of Molecular and Biomedical Science, The University of Adelaide, North Terrace, Adelaide, 5005, South Australia, Australia.
Thazin Nwe AungDepartment of Molecular and Biomedical Science, The University of Adelaide, North Terrace, Adelaide, 5005, South Australia, Australia.
Hanyuan ShenDepartment of Molecular and Biomedical Science, The University of Adelaide, North Terrace, Adelaide, 5005, South Australia, Australia.
Wei WangZhendong Australia - China Centre for Molecular Chinese Medicine, The University of Adelaide, North Terrace, Adelaide, 5005, South Australia, Australia.
David L AdelsonDepartment of Molecular and Biomedical Science, The University of Adelaide, North Terrace, Adelaide, 5005, South Australia, Australia. david.adelson@adelaide.edu.au.ORCID http://orcid.org/0000-0003-2404-5636
University of South Australia · AUThe University of Adelaide · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIn this report we examine candidate pathways perturbed by Compound Kushen Injection (CKI), a Traditional Chinese Medicine (TCM) that we have previously shown to alter the gene expression patterns of multiple pathways and induce apoptosis in cancer cells.

methodsWe have measured protein levels in Hep G2 and MDA-MB-231 cells for genes in the cell cycle pathway, DNA repair pathway and DNA double strand breaks (DSBs) previously shown to have altered expression by CKI. We have also examined energy metabolism by measuring [ADP]/[ATP] ratio (cell energy charge), lactate production and glucose consumption. Our results demonstrate that CKI can suppress protein levels for cell cycle regulatory proteins and DNA repair while increasing the level of DSBs. We also show that energy metabolism is reduced based on reduced glucose consumption and reduced cellular energy charge.

resultsOur results validate these pathways as important targets for CKI. We also examined the effect of the major alkaloid component of CKI, oxymatrine and determined that it had no effect on DSBs, a small effect on the cell cycle and increased the cell energy charge.

conclusionsOur results indicate that CKI likely acts through the effect of multiple compounds on multiple targets where the observed phenotype is the integration of these effects and synergistic interactions.

Indexed as

AlkaloidsAntineoplastic Agents, PhytogenicCell CycleCell Line, TumorDNA Breaks, Double-StrandedDNA RepairDrugs, Chinese HerbalEnergy MetabolismGene Expression Regulation, NeoplasticHumansMatrinesQuinolizinesSmilaxAlkaloidsAntineoplastic Agents, PhytogenicDrugs, Chinese HerbalkushenMatrinesoxymatrineQuinolizinesAlkaloidCell-cycleCyclinKu70Ku80Matrine

Identifiers

PMID30678652
PMCPMC6345000
OpenAlexW2951311935

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.