ArticlePloS one2019
The design and testing of mini-barcode markers in marine lobsters.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 25 citations in OpenAlex.
- Development of a mitochondrial mini-barcode and its application in metabarcoding for identification of leech in traditional Chinese medicine.Scientific reports · 2025Article
- Article
- Using a DNA mini-barcode within the ITS region to identify toxic Amanita in mushroom poisoning cases.Applied microbiology and biotechnology · 2024Article
- DNA barcoding in herbal medicine: Retrospective and prospective.Journal of pharmaceutical analysis · 2023Review
- Metabarcoding of ichthyoplankton communities associated with a highly dynamic shelf region of the southwest Indian Ocean.PloS one · 2023Article
- Identification of Phyllosoma Larvae of the LobstersZoological studies · 2023Article
- Development of Mini-Barcode Based on Chloroplast Genome and Its Application in Metabarcoding Molecular Identification of Chinese Medicinal Material RadixFrontiers in plant science · 2022Article
- A new record of the spiny lobster,Biodiversity data journal · 2022Article
- The complete chloroplast genome of Gleditsia sinensis and Gleditsia japonica: genome organization, comparative analysis, and development of taxon specific DNA mini-barcodes.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Full-length mitochondrial cytochrome c oxidase I (COI) sequence information from lobster phyllosoma larvae can be difficult to obtain when DNA is degraded or fragmented. Primers that amplify smaller fragments are also more useful in metabarcoding studies. In this study, we developed and tested a method to design a taxon-specific mini-barcode primer set for marine lobsters. The shortest, most informative portion of the COI gene region was identified in silico, and a DNA barcode gap analysis was performed to assess its reliability as species diagnostic marker. Primers were designed, and cross-species amplification success was tested on DNA extracted from a taxonomic range of spiny-, clawed-, slipper- and blind lobsters. The mini-barcode primers successfully amplified both adult and phyllosoma COI fragments, and were able to successfully delimit all species analyzed. Previously published universal primer sets were also tested and sometimes failed to amplify COI from phyllosoma samples. The newly designed taxon-specific mini-barcode primers will increase the success rate of species identification in bulk environmental samples and add to the growing DNA metabarcoding toolkit.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.