ArticleVirulence2019
Characterization of multiple type-VI secretion system (T6SS) VgrG proteins in the pathogenicity and antibacterial activity of porcine extra-intestinal pathogenic Escherichia coli.
Article in Virulence, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 43 citations in OpenAlex.
- From gut colonization to recurrence:Gut microbes · 2026Review
- Itaconic Acid Directly Binds to the Cysteine Residue of PurF: A Novel Mechanism for Inhibiting Purine Metabolism in Porcine PathogenicMicroorganisms · 2026Article
- Type VI secretion system degeneration accelerates intestinal epithelial cell death in Escherichia coli O157:H7.PLoS pathogens · 2026Article
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- Large-scale genomic analysis reveals the distribution and diversity of type VI secretion systems inmSystems · 2025Article
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- A Dual-Targeting T6SS DNase Drives Bacterial Antagonism and Eukaryotic Apoptosis via the cGAS-STING-TNF Axis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Virulence genes and phylogenetic analysis of antibiotic-resistantVeterinary world · 2025Article
- Population structure and antibiotic resistance of swine extraintestinal pathogenic Escherichia coli from China.Nature communications · 2024Article
- Baicalin Weakens the Virulence of Porcine Extraintestinal PathogenicBiomolecules · 2024Article
- Comparative genomics and host range analysis of four Ralstonia pseudosolanacearum strains isolated from sunflower reveals genomic and phenotypic differences.BMC genomics · 2024Article
- Genome-wide identification of genes critical forVirulence · 2023Article
- Distribution and diversity of type VI secretion system clusters inMicrobial genomics · 2023Article
- Comparative Genomic Analysis of a NovelMicroorganisms · 2023Article
- Bacterial type VI secretion system (T6SS): an evolved molecular weapon with diverse functionality.Biotechnology letters · 2023Review
- Identification of the BolA Protein Reveals a Novel Virulence Factor in K. pneumoniae That Contributes to Survival in Host.Microbiology spectrum · 2022Article
- A Quorum Sensing-Regulated Type VI Secretion System Containing Multiple Nonredundant VgrG Proteins Is Required for Interbacterial Competition in Chromobacterium violaceum.Microbiology spectrum · 2022Article
- Does Secondary Plant Metabolite Ursolic Acid Exhibit Antibacterial Activity against UropathogenicPharmaceutics · 2022Article
- T6SS Accessory Proteins, Including DUF2169 Domain-Containing Protein and Pentapeptide Repeats Protein, Contribute to Bacterial Virulence in T6SS Group_5 ofPlants (Basel, Switzerland) · 2021Article
- Function of Rhs proteins in porcine extraintestinal pathogenic Escherichia coli PCN033.Journal of microbiology (Seoul, Korea) · 2021Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Porcine extra-intestinal pathogenic Escherichia coli (ExPEC) causes great economic losses to the pig industry and poses a serious threat to public health worldwide. Some secreted virulence factors have been reported to be involved in the pathogenicity of the infection caused by ExPEC. Type-VI secretion system (T6SS) is discovered in many Gram-negative bacteria and contributes to the virulence of pathogenic bacteria. Valine-glycine repeat protein G (VgrG) has been reported as an important component of the functional T6SS. In our previous studies, a functional T6SS was identified in porcine ExPEC strain PCN033. Further analysis of the PCN033 genome identified two putative vgrGs genes (vgrG1 and 0248) located inside T6SS cluster and another two (vgrG2 and 1588) outside it. This study determined the function of the four putative VgrG proteins by constructing a series of mutants and complemented strains. In vitro, the VgrG1 protein was observed to be involved in the antibacterial ability and the interactions with cells. The animal model experiment showed that the deletion of vgrG1 significantly led to the decrease in the multiplication capacity of PCN033. However, the deletion of 0248 and/or the deletion of vgrG2 and 1588 had no effect on the pathogenicity of PCN033. The study of four putative VgrGs in PCN033 indicated that only VgrG1 plays an important role in the interaction between PCN033 and other bacteria or host cells. This study can provide a novel perspective to the pathogenesis of PCN033 and lay the foundation for discovering potential T6SS effectors.
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