Evidence map›Paper›PMID 30675636›Full record

ArticleApplied microbiology and biotechnology2019

Rapid micro-assays for amylolytic activities determination: customization and validation of the tests.

Monika Borkowska, Wojciech Białas, Monika Kubiak, Ewelina Celińska

Abstract readValidation Study
In one paragraph

Article in Applied microbiology and biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Synthesis of Secretory Proteins inInternational journal of molecular sciences · 2022
    Article
  6. Article
  7. Article
  8. Article
  9. Review
  10. Article
  11. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Monika BorkowskaDepartment of Biotechnology and Food Microbiology, Poznan University of Life Sciences, ul. Wojska Polskiego 48, 60-627, Poznań, Poland.
Wojciech BiałasDepartment of Biotechnology and Food Microbiology, Poznan University of Life Sciences, ul. Wojska Polskiego 48, 60-627, Poznań, Poland.
Monika KubiakDepartment of Biotechnology and Food Microbiology, Poznan University of Life Sciences, ul. Wojska Polskiego 48, 60-627, Poznań, Poland.
Ewelina CelińskaDepartment of Biotechnology and Food Microbiology, Poznan University of Life Sciences, ul. Wojska Polskiego 48, 60-627, Poznań, Poland. ewelina.celinska@up.poznan.pl.ORCID http://orcid.org/0000-0001-8372-8459

Funding

Ministerstwo Nauki i Szkolnictwa Wy?szego IP2015 011074
6 · The paper itself

Abstract

High-throughput function-based screening techniques remain the major bottleneck in the novel biocatalysts development pipeline. In the present study, we customized protocols for amylolytic activity determination (Somogyi-Nelson and starch-iodine tests) to micro-volume thermalcycler-based assays (linearity range 60-600 μM of reducing sugar, R

Indexed as

alpha-AmylasesCellulaseEnzyme AssaysGlucan 1,4-alpha-GlucosidaseHigh-Throughput Screening AssaysHot TemperatureStarchalpha-AmylasesCellulaseGlucan 1,4-alpha-Glucosidaseiodinated starchStarchAlpha-amylaseEnzymatic assayGlucoamylaseMicroassayMicro-volumeThermalcycler

Identifiers

PMID30675636
PMCPMC6420461

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.