Evidence map›Paper›PMID 30675134›Full record

ArticleBiological procedures online2019

Circular Estimate Method (CEM) - a Simple Method to Estimate

Marcelo Estrella Josende, Silvana Manske Nunes, Larissa Müller, Marlize Ferreira-Cravo, José Marìa Monserrat, Juliane Ventura-Lima

Abstract read
In one paragraph

Article in Biological procedures online, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Frontiers in nutrition · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Marcelo Estrella Josende1Instituto de Ciências Biológicas (ICB), Universidade Federal do Rio Grande - FURG, Av. Itália km 8, Rio Grande, RS 96203-900 Brazil.ORCID 0000-0002-2874-7152
Silvana Manske Nunes1Instituto de Ciências Biológicas (ICB), Universidade Federal do Rio Grande - FURG, Av. Itália km 8, Rio Grande, RS 96203-900 Brazil.
Larissa Müller1Instituto de Ciências Biológicas (ICB), Universidade Federal do Rio Grande - FURG, Av. Itália km 8, Rio Grande, RS 96203-900 Brazil.
Marlize Ferreira-Cravo1Instituto de Ciências Biológicas (ICB), Universidade Federal do Rio Grande - FURG, Av. Itália km 8, Rio Grande, RS 96203-900 Brazil.
José Marìa Monserrat1Instituto de Ciências Biológicas (ICB), Universidade Federal do Rio Grande - FURG, Av. Itália km 8, Rio Grande, RS 96203-900 Brazil.
Juliane Ventura-Lima1Instituto de Ciências Biológicas (ICB), Universidade Federal do Rio Grande - FURG, Av. Itália km 8, Rio Grande, RS 96203-900 Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundNematodes are used in many different fields of science, including environmental and biomedical research. Counting and/or estimating nematode numbers is required during research. Although being one of the most common procedures, this apparently simple task is a time-consuming process, prone to errors and concerns regarding procedure, reliability, and accuracy. When an estimate is necessary, there is a traditional manual counting procedure that in this study it will be called as "drop method" (DM). This popular method that extrapolates an animal count from a small drop of fluid shows a high coefficient of variation. To solve this problem, the present study used the free-living nematode

resultsThe new method showed a low coefficient of variation and a close relationship between estimated and real counts of the total number of nematodes in large

conclusionThe proposed method is accurate, facile and reproducible, requiring simple, inexpensive materials that make it an excellent alternative to the DM manual counting procedure. Although the DM is faster, its estimates are not as accurate or as precise as those of the new proposed method.

Indexed as

AccuracyCaenorhabditis elegansCountingEstimatingNematodePrecisionReliabilityReproducibility

Identifiers

PMID30675134
PMCPMC6334471

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.