ArticleScientific reports2019
Quorum sensing in Pseudomonas aeruginosa mediated by RhlR is regulated by a small RNA PhrD.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 48 citations in OpenAlex.
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- Crosstalk between inovirus core gene and accessory toxin-antitoxin system mediates polylysogeny.Nature communications · 2025Article
- Water-Soluble Sulfur-Ylide-Functionalized Polyacrylamides for Antibacterial Surface Applications.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Chemical profile, virtual screening, and virulence-inhibiting properties of Sphagneticola trilobata L. essential oils against Pseudomonas aeruginosa.Scientific reports · 2025Article
- The Role of Yinqiao Powder in ModulatingInfection and drug resistance · 2025Article
- Molecular Aspects of the Functioning of Pathogenic Bacteria Biofilm Based onInternational journal of molecular sciences · 2024Review
- Citrate cross-feeding bymBio · 2024Article
- Divergent molecular strategies drive evolutionary adaptation to competitive fitness in biofilm formation.The ISME journal · 2024Article
- Marine-Derived Furanones Targeting Quorum-Sensing Receptors inBioinformatics and biology insights · 2024Article
- Bioactive Compounds fromMolecules (Basel, Switzerland) · 2023Article
- Evaluation of extracellular electron transfer in Pseudomonas aeruginosa by co-expression of intermediate genes in NAD synthetase production pathway.World journal of microbiology & biotechnology · 2022Article
- Genome-wide analysis reveals a rhamnolipid-dependent modulation of flagellar genes in Pseudomonas aeruginosa PAO1.Current genetics · 2022Article
- The function of small RNA inPeerJ · 2022Article
- Strategies to prevent, curb and eliminate biofilm formation based on the characteristics of various periods in one biofilm life cycle.Frontiers in cellular and infection microbiology · 2022Review
- In Silico and In Vitro Screening of Antipathogenic Properties ofAntibiotics (Basel, Switzerland) · 2021Article
- Quorum sensing systems, related virulence factors, and biofilm formation in Pseudomonas aeruginosa isolated from fish.Archives of microbiology · 2021Article
- The Impact of an Efflux Pump Inhibitor on the Activity of Free and Liposomal Antibiotics againstPharmaceutics · 2021Article
- Coexistence of Virulence Factors and Efflux Pump Genes in Clinical Isolates ofInternational journal of microbiology · 2021Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas aeruginosa is a highly invasive human pathogen in spite of the absence of classical host specific virulence factors. Virulence factors regulated by quorum sensing (QS) in P. aeruginosa cause acute infections to shift to chronic diseases. Several small regulatory RNAs (sRNAs) mediate fine-tuning of bacterial responses to environmental signals and regulate quorum sensing. In this study, we show that the quorum sensing regulator RhlR is positively influenced upon over expression of the Hfq dependent small RNA PhrD in Pseudomonas. RhlR transcripts starting from two of the four different promoters have same sequence predicted to base pair with PhrD. Over expression of PhrD increased RhlR transcript levels and production of the biosurfactant rhamnolipid and the redox active pyocyanin pigment. A rhlR::lacZ translational fusion from one of the four promoters showed 2.5-fold higher expression and, a 9-fold increase in overall rhlR transcription was seen in the wild type when compared to the isogenic phrD disruption mutant. Expression, in an E. coli host background, of a rhlR::lacZ fusion in comparison to a construct that harboured a scrambled interaction region resulted in a 10-fold increase under phrD over expression. The interaction of RhlR-5'UTR with PhrD in E. coli indicated that this regulation could function without the involvement of any Pseudomonas specific proteins. Overall, this study demonstrates that PhrD has a positive effect on RhlR and its associated physiology in P. aeruginosa.
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