Evidence map›Paper›PMID 30674899›Full record

ArticleScientific reports2019

De novo assembly of Agave sisalana transcriptome in response to drought stress provides insight into the tolerance mechanisms.

Muhammad Bilal Sarwar, Zarnab Ahmad, Bushra Rashid, Sameera Hassan, Per L Gregersen, Maria De la O Leyva, Istvan Nagy, Torben Asp, Tayyab Husnain

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
7.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 56 citations in OpenAlex.

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  9. De Novo Transcriptome ofPlants (Basel, Switzerland) · 2022
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  12. The complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2021
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  17. Identification and validation of superior housekeeping gene(s) for qRT-PCR data normalization inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020
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  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Muhammad Bilal SarwarPlant Genomics Lab, Center of Excellence in Molecular Biology, University of the Punjab, 87-West Canal Bank Road Thokar Niaz Baig, Lahore, 53700, Pakistan.
Zarnab AhmadPlant Genomics Lab, Center of Excellence in Molecular Biology, University of the Punjab, 87-West Canal Bank Road Thokar Niaz Baig, Lahore, 53700, Pakistan.
Bushra RashidPlant Genomics Lab, Center of Excellence in Molecular Biology, University of the Punjab, 87-West Canal Bank Road Thokar Niaz Baig, Lahore, 53700, Pakistan. bushra.cemb@pu.edu.pk.ORCID 0000-0002-2076-9920
Sameera HassanPlant Genomics Lab, Center of Excellence in Molecular Biology, University of the Punjab, 87-West Canal Bank Road Thokar Niaz Baig, Lahore, 53700, Pakistan.
Per L GregersenDepartment of Molecular Biology and Genetics, Aarhus University, Forsøgsvej 1, Slagelse, Denmark.ORCID 0000-0001-8243-0976
Maria De la O LeyvaDepartment of Molecular Biology and Genetics, Aarhus University, Forsøgsvej 1, Slagelse, Denmark.
Istvan NagyDepartment of Molecular Biology and Genetics, Aarhus University, Forsøgsvej 1, Slagelse, Denmark.
Torben AspDepartment of Molecular Biology and Genetics, Aarhus University, Forsøgsvej 1, Slagelse, Denmark.
Tayyab HusnainPlant Genomics Lab, Center of Excellence in Molecular Biology, University of the Punjab, 87-West Canal Bank Road Thokar Niaz Baig, Lahore, 53700, Pakistan.
University of the Punjab · PKAarhus University · DK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Agave, monocotyledonous succulent plants, is endemic to arid regions of North America, exhibiting exceptional tolerance to their xeric environments. They employ various strategies to overcome environmental constraints, such as crassulacean acid metabolism, wax depositions, and protective leaf morphology. Genomic resources of Agave species have received little attention irrespective of their cultural, economic and ecological importance, which so far prevented the understanding of the molecular bases underlying their adaptations to the arid environment. In this study, we aimed to elucidate molecular mechanism(s) using transcriptome sequencing of A. sisalana. A de novo approach was applied to assemble paired-end reads. The expression study unveiled 3,095 differentially expressed unigenes between well-irrigated and drought-stressed leaf samples. Gene ontology and KEGG analysis specified a significant number of abiotic stress responsive genes and pathways involved in processes like hormonal responses, antioxidant activity, response to stress stimuli, wax biosynthesis, and ROS metabolism. We also identified transcripts belonging to several families harboring important drought-responsive genes. Our study provides the first insight into the genomic structure of A. sisalana underlying adaptations to drought stress, thus providing diverse genetic resources for drought tolerance breeding research.

Indexed as

AgaveGene Expression Regulation, PlantGene OntologyStress, PhysiologicalTranscriptomeDehydration

Identifiers

PMID30674899
PMCPMC6344536
OpenAlexW2913625268

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.