ArticleNature communications2019
In vivo proximity proteomics of nascent synapses reveals a novel regulator of cytoskeleton-mediated synaptic maturation.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed.
- Local translation couples synaptic activity to mitochondrial adaptation in dendrites.bioRxiv : the preprint server for biology · 2026Article
- Acute in vivo proximity labeling for membrane targeted proteomics in neuronal circuits.bioRxiv : the preprint server for biology · 2026Article
- Editorial: Impacts of cytoskeletal dysregulation on spinogenesis.Frontiers in cellular neuroscience · 2026Article
- A novel miniTurbo knock-in mouse reveals a protein interaction network of USP46 in the brain.Experimental animals · 2026Article
- Article
- Comparative Single-Cell Transcriptome Analysis of c-Met Receptor Expressing and Non-Expressing Projection Neurons in the Developing Frontal and Visual Cortices.Developmental neuroscience · 2025Article
- Bridging molecular and cellular neuroscience with proximity labeling technologies.Experimental & molecular medicine · 2025Review
- Sex and Depot Specific Adipocyte Proteome Profiling In Vivo via Intracellular Proximity Labeling.Comprehensive Physiology · 2025Article
- Understanding the molecular diversity of synapses.Nature reviews. Neuroscience · 2025Review
- Cross-Species Proximity-Dependent Protein Biotinylation: A Standardized Approach for Mapping Proxeomes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Proximity labeling uncovers the synaptic proteome under physiological and pathological conditions.Frontiers in cellular neuroscience · 2025Review
- Article
- Neuron type-specific proteomics reveals distinct Shank3 proteoforms in iSPNs and dSPNs lead to striatal synaptopathy in Shank3BMolecular psychiatry · 2024Article
- Molecular recording of calcium signals via calcium-dependent proximity labeling.Nature chemical biology · 2024Article
- Article
- Synaptic proteomics decode novel molecular landscape in the brain.Frontiers in molecular neuroscience · 2024Review
- Profiling Mouse Brain Single-Cell-Type Proteomes Via Adeno-Associated Virus-Mediated Proximity Labeling and Mass Spectrometry.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Single-cell synaptome mapping: its technical basis and applications in critical period plasticity research.Frontiers in neural circuits · 2024Review
- Flotillin-1 enhances radioresistance through reducing radiation-induced DNA damage and promoting immune escape via STING signaling pathway in non-small cell lung cancer.Cancer biology & therapy · 2023Article
- Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
Abstract
Excitatory synapse formation during development involves the complex orchestration of both structural and functional alterations at the postsynapse. However, the molecular mechanisms that underlie excitatory synaptogenesis are only partially resolved, in part because the internal machinery of developing synapses is largely unknown. To address this, we apply a chemicogenetic approach, in vivo biotin identification (iBioID), to discover aspects of the proteome of nascent synapses. This approach uncovered sixty proteins, including a previously uncharacterized protein, CARMIL3, which interacts in vivo with the synaptic cytoskeletal regulator proteins SrGAP3 (or WRP) and actin capping protein. Using new CRISPR-based approaches, we validate that endogenous CARMIL3 is localized to developing synapses where it facilitates the recruitment of capping protein and is required for spine structural maturation and AMPAR recruitment associated with synapse unsilencing. Together these proteomic and functional studies reveal a previously unknown mechanism important for excitatory synapse development in the developing perinatal brain.
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