ArticleProceedings of the National Academy of Sciences of the United States of America2019
Optofluidic real-time cell sorter for longitudinal CTC studies in mouse models of cancer.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 61 citations in OpenAlex.
- Microtubule polymerization generates microtentacles important in circulating tumor cell invasion.Biophysical journal · 2025Article
- Application of Liquid Biopsy Technology in Lung Cancer: A Bibliometric Study and Visualization Analysis.Cancer management and research · 2025Review
- Leukemia circulation kinetics revealed through blood exchange method.Communications biology · 2024Article
- Deep learning-enabled detection of rare circulating tumor cell clusters in whole blood using label-free, flow cytometry.Lab on a chip · 2024Article
- Deciphering the Biology of Circulating Tumor Cells through Single-Cell RNA Sequencing: Implications for Precision Medicine in Cancer.International journal of molecular sciences · 2023Review
- Dissecting metastasis using preclinical models and methods.Nature reviews. Cancer · 2023Review
- Optical Detection of Cancer Cells Using Lab-on-a-Chip.Biosensors · 2023Review
- Trends and Potential of Machine Learning and Deep Learning in Drug Study at Single-Cell Level.Research (Washington, D.C.) · 2023Review
- nuPRISM: Microfluidic Genome-Wide Phenotypic Screening Platform for Cellular Nuclei.ACS central science · 2022Article
- Isolation of circulating tumor cells.iScience · 2022Review
- A microfluidic platform enables comprehensive gene expression profiling of mouse retinal stem cells.Lab on a chip · 2021Article
- Measuring kinetics and metastatic propensity of CTCs by blood exchange between mice.Nature communications · 2021Article
- A data-driven approach to modeling cancer cell mechanics during microcirculatory transport.Scientific reports · 2021Article
- Single-cell RNA sequencing in cancer: Applications, advances, and emerging challenges.Molecular therapy oncolytics · 2021Review
- Dive into Single, Seek Out Multiple: Probing Cancer Metastases via Single-Cell Sequencing and Imaging Techniques.Cancers · 2021Review
- Hydrodynamic assisted multiparametric particle spectrometry.Scientific reports · 2021Article
- Single-Cell Sequencing and Organoids: A Powerful Combination for Modelling Organ Development and Diseases.Reviews of physiology, biochemistry and pharmacology · 2021Review
- Detection Methods and Clinical Applications of Circulating Tumor Cells in Breast Cancer.Frontiers in oncology · 2021Review
- Single-cell analyses of aging, inflammation and senescence.Ageing research reviews · 2020Review
- YAP Enhances Tumor Cell Dissemination by Promoting Intravascular Motility and Reentry into Systemic Circulation.Cancer research · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
24 authors at 3 institutions in 1 country.
Funding
Abstract
Circulating tumor cells (CTCs) play a fundamental role in cancer progression. However, in mice, limited blood volume and the rarity of CTCs in the bloodstream preclude longitudinal, in-depth studies of these cells using existing liquid biopsy techniques. Here, we present an optofluidic system that continuously collects fluorescently labeled CTCs from a genetically engineered mouse model (GEMM) for several hours per day over multiple days or weeks. The system is based on a microfluidic cell sorting chip connected serially to an unanesthetized mouse via an implanted arteriovenous shunt. Pneumatically controlled microfluidic valves capture CTCs as they flow through the device, and CTC-depleted blood is returned back to the mouse via the shunt. To demonstrate the utility of our system, we profile CTCs isolated longitudinally from animals over 4 days of treatment with the BET inhibitor JQ1 using single-cell RNA sequencing (scRNA-Seq) and show that our approach eliminates potential biases driven by intermouse heterogeneity that can occur when CTCs are collected across different mice. The CTC isolation and sorting technology presented here provides a research tool to help reveal details of how CTCs evolve over time, allowing studies to credential changes in CTCs as biomarkers of drug response and facilitating future studies to understand the role of CTCs in metastasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.