ReviewPlant cell reports2019
CRISPR/Cas-mediated gene targeting in plants: finally a turn for the better for homologous recombination.
Review in Plant cell reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
65 citing papers in PubMed, 145 citations in OpenAlex.
- Detection of CRISPR-Cas-induced mutations in Daphnia.G3 (Bethesda, Md.) · 2026Article
- AI-enhanced framework for optimizing CRISPR-Cas gene editing in crop biotechnology addressing regulatory challenges and opportunities in global agricultural practices.Frontiers in plant science · 2026Article
- Detection of CRISPR-Cas-induced mutations inbioRxiv : the preprint server for biology · 2025Article
- The evolving landscape of precise DNA insertion in plants.Nature communications · 2025Review
- Multiplex CRISPR-Cas9 editing of chlorophyll biosynthesis genes in chickpea via protoplast and Agrobacterium-mediated transformation.Functional & integrative genomics · 2025Article
- Plant conservation in the age of genome editing: opportunities and challenges.Genome biology · 2024Review
- Article
- High-yield BMP2 expression in rice cells via CRISPR and endogenous αAmy3 promoter.Applied microbiology and biotechnology · 2024Article
- Enhancement of specialized metabolites using CRISPR/Cas gene editing technology in medicinal plants.Frontiers in plant science · 2024Review
- The economics and policy of genome editing in crop improvement.The plant genome · 2023Review
- CRISPR-Based Genome Editing Tools: An Accelerator in Crop Breeding for a Changing Future.International journal of molecular sciences · 2023Review
- Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein and hairy roots: a perfect match for gene functional analysis and crop improvement.Current opinion in biotechnology · 2023Review
- Strategies for delivery of CRISPR/Cas-mediated genome editing to obtain edited plants directly without transgene integration.Frontiers in genome editing · 2023Review
- Improving Precise Genome Editing Using Donor DNA/gRNA Hybrid Duplex Generated by Complementary Bases.Biomolecules · 2022Article
- Updated scientific opinion on plants developed through cisgenesis and intragenesis.EFSA journal. European Food Safety Authority · 2022Article
- CRISPR/Cas9-Mediated Targeted DNA Integration: Rearrangements at the Junction of Plant and Plasmid DNA.International journal of molecular sciences · 2022Article
- Optimization of Genome Knock-In Method: Search for the Most Efficient Genome Regions for Transgene Expression in Plants.International journal of molecular sciences · 2022Review
- Efficient production of transgene-free, gene-edited carrot plants via protoplast transformation.Plant cell reports · 2022Article
- Nonhomologous end joining as key to CRISPR/Cas-mediated plant chromosome engineering.Plant physiology · 2022Article
- From Genome Sequencing to CRISPR-Based Genome Editing for Climate-Resilient Forest Trees.International journal of molecular sciences · 2022Review
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
key messageWe summarize recent progress of CRISPR/Cas9-mediated gene targeting in plants, provide recommendations for designing gene-targeting vectors and highlight the potential of new technologies applicable to plants. Gene targeting (GT) is a tool of urgent need for plant biotechnology and breeding. It is based on homologous recombination that is able to precisely introduce desired modifications within a target locus. However, its low efficiency in higher plants is a major barrier for its application. Using site-specific nucleases, such as the recent CRISPR/Cas system, GT has become applicable in plants, via the induction of double-strand breaks, although still at a too low efficiency for most practical applications in crops. Recently, a variety of promising new improvements regarding the efficiency of GT has been reported by several groups. It turns out that GT can be enhanced by cell-type-specific expression of Cas nucleases, by the use of self-amplified GT-vector DNA or by manipulation of DNA repair pathways. Here, we highlight the most recent progress of GT in plants. Moreover, we provide suggestions on how to use the technology efficiently, based on the mechanisms of DNA repair, and highlight several of the newest GT strategies in yeast or mammals that are potentially applicable to plants. Using the full potential of GT technology will definitely help us pave the way in enhancing crop yields and food safety for an ecologically friendly agriculture.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.