ArticlePlant molecular biology2019
GhCyP3 improves the resistance of cotton to Verticillium dahliae by inhibiting the E3 ubiquitin ligase activity of GhPUB17.
Article in Plant molecular biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- E3 ubiquitin ligases in plant defense: mechanisms and prospects for resistance breeding.Science China. Life sciences · 2026Review
- Secreted Protein VdCUE Modulates Virulence ofJournal of fungi (Basel, Switzerland) · 2025Article
- Interaction Between Glycoside Hydrolase FsGH28c fromInternational journal of molecular sciences · 2025Article
- Genome-wide identification and expression analysis of the U-box gene family related to biotic and abiotic stresses in Coffea canephora L.BMC genomics · 2024Article
- The RING-Type E3 Ubiquitin Ligase GenePlants (Basel, Switzerland) · 2024Article
- Transcriptome analysis of Gossypium hirsutum cultivar Zhongzhimian No.2 uncovers the gene regulatory networks involved in defense against Verticillium dahliae.BMC plant biology · 2024Article
- A putative E3 ubiquitin ligase substrate receptor degrades transcription factor SmNAC to enhance bacterial wilt resistance in eggplant.Horticulture research · 2024Article
- Insights to Gossypium defense response against Verticillium dahliae: the Cotton Cancer.Functional & integrative genomics · 2023Review
- Defense Mechanisms of CottonInternational journal of molecular sciences · 2022Review
- Analysis of theGenes · 2022Article
- How Many Faces Does the Plant U-Box E3 Ligase Have?International journal of molecular sciences · 2022Review
- Verticillium dahliae effector VDAL protects MYB6 from degradation by interacting with PUB25 and PUB26 E3 ligases to enhance Verticillium wilt resistance.The Plant cell · 2021Article
- Evolutionary and Characteristic Analysis of RING-DUF1117 E3 Ubiquitin Ligase Genes inBiomolecules · 2021Article
- Cerium oxide nanoparticles improve cotton salt tolerance by enabling better ability to maintain cytosolic KJournal of nanobiotechnology · 2021Article
- Regulatory Network of Cotton Genes in Response to Salt, Drought and Wilt Diseases (Frontiers in plant science · 2021Review
- Genome-wide association analysis reveals loci and candidate genes involved in fiber quality traits in sea island cotton (Gossypium barbadense).BMC plant biology · 2020Article
- An Overview of the Molecular Genetics of Plant Resistance to the Verticillium Wilt PathogenInternational journal of molecular sciences · 2020Review
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Authors and funding
8 authors at 2 institutions in 2 countries.
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Abstract
key messageA U-box E3 ubiquitin ligase GhPUB17 is inhibited by GhCyP3 with antifungal activity and acts as a negative regulator involved in cotton resistance to Verticillium dahliae. E3 ubiquitin ligases, the key component enzymes of the ubiquitin-proteasome system, which contains the most diverse structural and functional members involved in the determination of target specificity and the regulation of metabolism, have been well documented in previous studies. Here, we identify GhPUB17, a U-box E3 ligase in cotton that has ubiquitination activity and is involved in the cotton immune response to Verticillium dahliae. The expression level of GhPUB17 is downregulated in the ssn mutant with a constitutively activated immune response (Sun et al., Nat Commun 5:5372, 2014). Infection with V. dahliae or exogenous hormone treatment, including jasmonic acid and salicylic acid, significantly upregulated GhPUB17 in cotton roots, which suggested a possible role for this E3 ligase in the plant immune response to pathogens. Moreover, GhPUB17-knockdown cotton plants are more resistant to V. dahliae, whereas GhPUB17-overexpressing plants are more susceptible to the pathogen, which indicated that GhPUB17 is a negative regulator of cotton resistance to V. dahliae. A yeast two-hybrid (Y2H) assay identified GhCyP3 as a protein that interacts with GhPUB17, and this finding was confirmed by further protein interaction assays. The downregulation of GhCyP3 in cotton seedlings attenuated the plants' resistance to V. dahliae. In addition, GhCyP3 showed antifungal activity against V. dahliae, and the E3 ligase activity of GhPUB17 was repressed by GhCyP3 in vitro. These results suggest that GhPUB17 negatively regulates cotton immunity to V. dahliae and that the antifungal protein GhCyP3 likely interacts with and inhibits the ligase activity of GhPUB17 and plays an important role in the cotton-Verticillium interaction.
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