ArticleJournal of materials science. Materials in medicine2019
A bioactive nano-calcium phosphate paste for in-situ transfection of BMP-7 and VEGF-A in a rabbit critical-size bone defect: results of an in vivo study.
Article in Journal of materials science. Materials in medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Microvascular anatomy and mapping of intraosseous arterioles in the human L1 vertebral body: a histological study, literature comparison, and relevance for fracture healing.Journal of spine surgery (Hong Kong) · 2026Article
- Anatomy of the intraosseous arterial microvasculature of the adult lumbar vertebral body and its role in fracture healing: a literature review and historical perspective.Journal of spine surgery (Hong Kong) · 2026Review
- Biomaterial-assisted gene therapy for bone repair.Materials today. Bio · 2026Review
- Nano Biomaterials in Drug Delivery and Tissue Engineering.Current drug delivery · 2026Review
- Vascular anatomy of L1 vertebral body in Wistar rat-corrosion-fluorescence, diaphanization and histological analysis, comparison to humans, and importance in blood supply-related investigation.Journal of spine surgery (Hong Kong) · 2025Article
- The healing process of vertebral body fracture in Wistar rats: creation of an animal model and demonstration of the impact of anterolateral vascularization disruption on bone healing.Journal of spine surgery (Hong Kong) · 2025Article
- Global Research Trends and Hotspots in Fracture Nonunion and Delayed Union: A 2-Decade Bibliometric Analysis.Indian journal of orthopaedics · 2025Article
- A particle-filled hydrogel based on alginate and calcium phosphate nanoparticles as bone adhesive.Journal of materials science. Materials in medicine · 2024Article
- Review
- Development of triple-functionalized calcium phosphate nanoparticles as an advanced drug delivery system for bone tissue repair.Regenerative therapy · 2024Article
- Structural DNA nanotechnology at the nexus of next-generation bio-applications: challenges and perspectives.Nanoscale advances · 2024Review
- Recent advancement in vascularized tissue-engineered bone based on materials design and modification.Materials today. Bio · 2023Review
- Evaluation of How Methacrylate Gelatin Hydrogel Loaded withJournal of functional biomaterials · 2023Article
- Functionalized calcium phosphate nanoparticles to direct osteoprotegerin to bone lesion sites in a medaka (Frontiers in endocrinology · 2023Article
- Bone healing study of alendronate combined with enoxaparin sodium bone cement in rabbits with bone defects.Journal of orthopaedic surgery and research · 2022Article
- Bone Sialoprotein Immobilized in Collagen Type I Enhances Bone Regeneration In vitro and In vivo.International journal of bioprinting · 2022Article
- The biological applications of DNA nanomaterials: current challenges and future directions.Signal transduction and targeted therapy · 2021Review
- Biological and Medical Applications of Calcium Phosphate Nanoparticles.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Review
- Synthesis and characterization of PLGA/HAP scaffolds with DNA-functionalised calcium phosphate nanoparticles for bone tissue engineering.Journal of materials science. Materials in medicine · 2020Article
- Biomaterials for In Situ Tissue Regeneration: A Review.Biomolecules · 2019Review
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
The aim of this study was to prepare an injectable DNA-loaded nano-calcium phosphate paste that is suitable as bioactive bone substitution material. For this we used the well-known potential of calcium phosphate in bone contact and supplemented it with DNA for the in-situ transfection of BMP-7 and VEGF-A in a critical-size bone defect. 24 New Zealand white rabbits were randomly divided into two groups: One group with BMP-7- and VEGF-A-encoding DNA on calcium phosphate nanoparticles and a control group with calcium phosphate nanoparticles only. The bone defect was created at the proximal medial tibia and filled with the DNA-loaded calcium phosphate paste. As control, a bone defect was filled with the calcium phosphate paste without DNA. The proximal tibia was investigated 2, 4 and 12 weeks after the operation. A histomorphological analysis of the dynamic bone parameters was carried out with the Osteomeasure system. The animals treated with the DNA-loaded calcium phosphate showed a statistically significantly increased bone volume per total volume after 4 weeks in comparison to the control group. Additionally, a statistically significant increase of the trabecular number and the number of osteoblasts per tissue area were observed. These results were confirmed by radiological analysis. The DNA-loaded bone paste led to a significantly faster healing of the critical-size bone defect in the rabbit model after 4 weeks. After 12 weeks, all defects had equally healed in both groups. No difference in the quality of the new bone was found. The injectable DNA-loaded calcium phosphate paste led to a faster and more sustained bone healing and induced an accelerated bone formation after 4 weeks. The material was well integrated into the bone defect and new bone was formed on its surface. The calcium phosphate paste without DNA led to a regular healing of the critical-size bone defect, but the healing was slower than the DNA-loaded paste. Thus, the in-situ transfection with BMP-7 and VEGF-A significantly improved the potential of calcium phosphate as pasty bone substitution material.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.