ArticlePlant reproduction2019
A simple and robust protocol for immunostaining Arabidopsis pollen nuclei.
Article in Plant reproduction, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 16 citations in OpenAlex.
- Dynamic changes in chromatin structure and transcriptional activity in the generative cells of Lilium longiflorum.Journal of plant research · 2025Article
- H3K36 methylation stamps transcription resistive to preserve development in plants.Nature plants · 2025Article
- Regulation of cell cycle in plant gametes: when is the right time to divide?Development (Cambridge, England) · 2025Review
- DDM1-mediated R-loop resolution and H2A.Z exclusion facilitates heterochromatin formation in Arabidopsis.Science advances · 2023Article
- Polycomb-mediated repression of paternal chromosomes maintains haploid dosage in diploid embryos ofeLife · 2022Article
- The IBI1 Receptor of β-Aminobutyric Acid Interacts with VOZ Transcription Factors to Regulate Abscisic Acid Signaling and Callose-Associated Defense.Molecular plant · 2020Article
- Targeted reprogramming of H3K27me3 resets epigenetic memory in plant paternal chromatin.Nature cell biology · 2020Article
- Chromatin Organization in Early Land Plants Reveals an Ancestral Association between H3K27me3, Transposons, and Constitutive Heterochromatin.Current biology : CB · 2020Article
- Impact of phenanthrene on primary metabolite profiling in root exudates and maize mucilage.Environmental science and pollution research international · 2020Article
- Profiling of main metabolites in root exudates and mucilage collected from maize submitted to cadmium stress.Environmental science and pollution research international · 2019Article
- Special issue: cellular omics methods in plant reproduction research.Plant reproduction · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Pollen represents the male sexual lineage in flowering plants. At maturity, pollen grains are composed of a companion vegetative cell with embedded sperm. During pollen development, these two cell types acquire vastly differing cell fates. Underlying this differential fate acquisition is dramatic reconfiguration of pollen chromatin that is highly evident at a cytological level. The precise link between histone mark deposition and fate acquisition remains largely unexplored, which in part has been hindered by the difficulty in working with pollen in model plant species like Arabidopsis. Here, we describe a simple and robust protocol to isolate Arabidopsis pollen nuclei and immunostain for histone marks. Plant growth aside, the protocol can be performed over 2 days with few Arabidopsis plants, thus allowing multiple genotypes to be analysed in parallel. We also describe a method to de-mask epitopes through antigen retrieval, which vastly improves the signal for antibodies that target heterochromatic histone marks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.