ArticleViruses2019
Establishment of an Alphavirus-Specific Neutralization Assay to Distinguish Infections with Different Members of the Semliki Forest complex.
Article in Viruses, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 66 citations in OpenAlex.
- Article
- Facile quantitative diagnostic testing for neutralizing antibodies against Chikungunya virus.BMC infectious diseases · 2024Article
- Article
- Chikungunya virus antepartum transmission and abnormal infant outcomes in a cohort of pregnant women in Nigeria.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2024Article
- Performance Evaluation of VIDASDiagnostics (Basel, Switzerland) · 2023Article
- Susceptibility of Aedes albopictus, Ae. aegypti and human populations to Ross River virus in Kuala Lumpur, Malaysia.PLoS neglected tropical diseases · 2023Article
- A Bivalent Trans-Amplifying RNA Vaccine Candidate Induces Potent Chikungunya and Ross River Virus Specific Immune Responses.Vaccines · 2022Article
- Preparation and application of chikungunya pseudovirus containing double reporter genes.Scientific reports · 2022Article
- A taRNA vaccine candidate induces a specific immune response that protects mice against Chikungunya virus infections.Molecular therapy. Nucleic acids · 2022Article
- Article
- The SARS-CoV-2 Variant Omicron Is Able to Escape Vaccine-Induced Humoral Immune Responses, but Is Counteracted by Booster Vaccination.Vaccines · 2022Article
- Article
- Comparative Investigation of Methods for Analysis of SARS-CoV-2-Spike-Specific Antisera.Viruses · 2022Article
- Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever.Scientific reports · 2021Article
- Development of a Sensitive Detection Method for Alphaviruses and Its Use as a Virus Neutralization Assay.Viruses · 2021Article
- The green tea catechin epigallocatechin gallate inhibits SARS-CoV-2 infection.The Journal of general virology · 2021Article
- ArthritogenicLife (Basel, Switzerland) · 2021Review
- Comparison of potency assays to assess SARS-CoV-2 neutralizing antibody capacity in COVID-19 convalescent plasma.Journal of virological methods · 2021Article
- More Than Just Gene Therapy Vectors: Lentiviral Vector Pseudotypes for Serological Investigation.Viruses · 2021Review
- Incidence of Sindbis Virus in Hospitalized Patients With Acute Fevers of Unknown Cause in South Africa, 2019-2020.Frontiers in microbiology · 2021Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 4 countries.
Funding
Abstract
backgroundAlphaviruses are transmitted by arthropod vectors and can be found worldwide. Alphaviruses of the Semliki Forest complex such as chikungunya virus (CHIKV), Mayaro virus (MAYV) or Ross River virus (RRV) cause acute febrile illness and long-lasting arthralgia in humans, which cannot be clinically discriminated from a dengue virus or Zika virus infection. Alphaviruses utilize a diverse array of mosquito vectors for transmission and spread. For instance, adaptation of CHIKV to transmission by Aedes albopictus has increased its spread and resulted in large outbreaks in the Indian Ocean islands. For many alphaviruses commercial diagnostic tests are not available or show cross-reactivity among alphaviruses. Climate change and globalization will increase the spread of alphaviruses and monitoring of infections is necessary and requires virus-specific methods.
methodWe established an alphavirus neutralization assay in a 384-well format by using pseudotyped lentiviral vectors.
resultsMAYV-specific reactivity could be discriminated from CHIKV reactivity. Human plasma from blood donors infected with RRV could be clearly identified and did not cross-react with other alphaviruses.
conclusionThis safe and easy to use multiplex assay allows the discrimination of alphavirus-specific reactivity within a single assay and has potential for epidemiological surveillance. It might also be useful for the development of a pan-alphavirus vaccine.
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