Evidence map›Paper›PMID 30668904›Full record

ArticleBiochemistry2019

Ubiquitin Chains Bearing Genetically Encoded Photo-Cross-Linkers Enable Efficient Covalent Capture of (Poly)ubiquitin-Binding Domains.

Courtney N Braxton, Evan Quartner, Westley Pawloski, David Fushman, T Ashton Cropp

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 15 citations in OpenAlex.

  1. Synthetic Strategies for Activity-Based Probes to Decode Ubiquitin-Like Modifiers.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026
    Review
  2. Article
  3. Article
  4. Internal Ubiquitin Electrophiles for Covalent Trapping and Inhibition of Deubiquitinases.Chembiochem : a European journal of chemical biology · 2025
    Article
  5. Review
  6. Reading and Writing the Ubiquitin Code Using Genetic Code Expansion.Chembiochem : a European journal of chemical biology · 2024
    Review
  7. Article
  8. Article
  9. Review
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Courtney N BraxtonDepartment of Chemistry , Virginia Commonwealth University , 1001 West Main Street , P.O. Box 842006, Richmond , Virginia 23284-2006 , United States.
Evan QuartnerDepartment of Chemistry and Biochemistry, Center for Biomolecular Structure and Organization , University of Maryland , College Park , Maryland 20742-3360 , United States.
Westley PawloskiDepartment of Chemistry and Biochemistry, Center for Biomolecular Structure and Organization , University of Maryland , College Park , Maryland 20742-3360 , United States.
David FushmanDepartment of Chemistry and Biochemistry, Center for Biomolecular Structure and Organization , University of Maryland , College Park , Maryland 20742-3360 , United States.ORCID 0000-0002-6634-8056
T Ashton CroppDepartment of Chemistry , Virginia Commonwealth University , 1001 West Main Street , P.O. Box 842006, Richmond , Virginia 23284-2006 , United States.ORCID 0000-0003-1562-0176
University of Maryland, College Park · USVirginia Commonwealth University · US

Funding

Molecular Binding Partners of Non-canonical Ubiquitin ChainsR21NS093454 · NINDS · VIRGINIA COMMONWEALTH UNIVERSITY · PI CROPP, THOMAS ASHTON · 2016 to 2017
$421k
Howard Hughes Medical InstituteNINDS NIH HHS R21 NS093454
6 · The paper itself

Abstract

Ubiquitin-mediated signaling pathways regulate essentially every aspect of cell biology in eukaryotes. Ubiquitin receptors typically contain ubiquitin-binding domains (UBDs) that have the ability to recognize monomeric ubiquitin (Ub) and polymeric Ub (polyUb) chains. However, how signaling specificity is achieved remains poorly understood, and many of the UBDs that selectively recognize polyUb chains of particular linkages still need to be identified and characterized. Here we report the incorporation of a genetically encoded photo-cross-linker, p-benzoyl-l-phenylalanine (Bpa), into recombinant Ub and enzymatically synthesized polyUb chains. This allows photo-cross-linking (covalent bond formation) of monoUb and K48- and K63-linked diUb chains to UBDs. This approach provides a framework for understanding Ub cellular signaling through the capture and identification of (poly)Ub-binding proteins.

Indexed as

BenzophenonesBinding SitesCarrier ProteinsCross-Linking ReagentsDNA-Binding ProteinsHistone ChaperonesMutationNuclear ProteinsPhenylalaninePolyubiquitinProteasome Endopeptidase ComplexProtein DomainsRNA, Transfer, TyrSaccharomyces cerevisiae ProteinsUbiquitinUbiquitin-Activating Enzymes4-benzoylphenylalanineBenzophenonesCarrier ProteinsCross-Linking ReagentsDNA-Binding ProteinsHistone ChaperonesNuclear ProteinsPhenylalaninePolyubiquitinProteasome Endopeptidase ComplexRNA, Transfer, TyrRPN1 protein, S cerevisiaeSaccharomyces cerevisiae ProteinsUBA2 protein, humanUbiquitinUbiquitin-Activating EnzymesUIMC1 protein, human

Identifiers

PMID30668904
PMCPMC6699165
OpenAlexW2913516597

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.