Evidence map›Paper›PMID 30666404›Full record

ArticleGraefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie2019

HMG-CoA reductase expression in human eyelid tissue and in a human meibomian gland epithelial cell line.

Kenneth G-J Ooi, Anupam Rao, Jonathan S-K Goh, Gary Gracie, Svetlana Cherepanoff, Michele C Madigan, Stephanie L Watson

Abstract read
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In one paragraph

Article in Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.1field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Kenneth G-J OoiSave Sight Institute, University of Sydney, Sydney Eye Hospital Campus, 8 Macquarie Street, Sydney, NSW, 2000, Australia. kgjooi@yahoo.com.au.ORCID http://orcid.org/0000-0001-8060-9374
Anupam RaoSave Sight Institute, University of Sydney, Sydney Eye Hospital Campus, 8 Macquarie Street, Sydney, NSW, 2000, Australia.
Jonathan S-K GohSave Sight Institute, University of Sydney, Sydney Eye Hospital Campus, 8 Macquarie Street, Sydney, NSW, 2000, Australia.
Gary GracieSydPath, St Vincent's Pathology Department, St Vincent's Hospital, 390 Victoria St, Darlinghurst, NSW, 2010, Australia.
Svetlana CherepanoffUniversity of Sydney, Sydney, 2006, Australia.
Michele C MadiganSave Sight Institute, University of Sydney, Sydney Eye Hospital Campus, 8 Macquarie Street, Sydney, NSW, 2000, Australia.
Stephanie L WatsonSave Sight Institute, University of Sydney, Sydney Eye Hospital Campus, 8 Macquarie Street, Sydney, NSW, 2000, Australia.
The University of Sydney · AUSt Vincent's Hospital Sydney · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purpose3-Hydroxy-3-methyl-glutaryl-coenzyme A reductase (HMGCR), the rate-limiting enzyme of cholesterol production, has been found to contribute to lipid secretion from skin sebaceous glands and hair follicles. We assessed for HMGCR expression in human eyelid tissue and in immortalized human meibomian gland epithelial cells (HMGECs) using immunohistochemistry.

methodsFull thickness human eyelid specimens in archival paraffin blocks were obtained. A section from each block was stained with hematoxylin and eosin and examined by an ocular pathologist for validation of tissue pathology. Immunohistochemistry was performed using rabbit anti-human HMGCR antibody on serial sections using the Ventana automated staining system. HMGCR expression was examined for in HMEGCs with fluorescence immunocytochemistry and confocal microscopy.

resultsThirteen full thickness eyelid specimens met the inclusion criteria. All specimens contained meibomian glands, and 2 (15%) contained glands of Zeis, 3 (23%) pilosebaceous glands, 2 (15%), accessory lacrimal glands, and 2 (15%), glands of Moll, respectively. Immunohistochemistry showed HMGCR expression in meibocytes of meibomian glands and sebocytes of Zeis and pilosebaceous glands in all specimens. HMGCR expression was also evident in vascular endothelium. Immunofluorescence was positive for HMGCR expression on HMGEC cells. No labeling was seen for the negative Ig control.

conclusionHMGCR was expressed in all eyelid sebaceous-type glands and in HMGECs, consistent with a role for cholesterol production in the genesis of tear film lipids. The observed expression also provides a rationale for using topical statins, inhibitors of HMGCR, as novel tear film lipid stabilizers in conditions such as blepharitis, where meibum production is aberrant.

Indexed as

AdultAgedAged, 80 and overBasal Cell CarcinomaCarcinoma, Squamous CellCell LineEpithelial CellsEyelid NeoplasmsFemaleFluorescent Antibody Technique, IndirectHumansHydroxymethylglutaryl CoA ReductasesImmunohistochemistryMaleMeibomian GlandsMicroscopy, ConfocalHMGCR protein, humanHydroxymethylglutaryl CoA ReductasesBlepharitisCholesterolDry eyeHMG-CoA reductaseMeibomian glandStatinVascular endothelium

Identifiers

PMID30666404
OpenAlexW2911663284

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.