ArticleDevelopment (Cambridge, England)2019
Broad applicability of a streamlined ethyl cinnamate-based clearing procedure.
Article in Development (Cambridge, England), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
80 citing papers in PubMed.
- Spatiotemporal control of STING activation and saRNA delivery decouples humoral and cellular immunity.bioRxiv : the preprint server for biology · 2026Article
- Label-free biochemical imaging and time point analysis of neural organoids via deep learning-enhanced Raman microspectroscopy.Science advances · 2026Article
- A uniform tissue-clearing framework and mesoSPIM-ultra enable cm-scale single-neuron tracing.bioRxiv : the preprint server for biology · 2026Article
- Aging disrupts sympathetic innervation of the thymus.Cell reports · 2026Article
- Generation of In Vivo-Inspired 3D Collagen Models for Guided Tumor Invasion In Vitro.Current protocols · 2026Article
- Rapid 3D Immunolabeling and Light Sheet Microscopy for Quantitative Analysis of Intact Tissues.Computational and structural biotechnology journal · 2026Article
- Ethyl Cinnamate-Based Tissue Clearing Technique on Vocal Fold Mucosa with Vasculature Model.Journal of voice : official journal of the Voice Foundation · 2025Article
- Optimized toolkit for the manipulation of immortalized axolotl fibroblasts.Methods (San Diego, Calif.) · 2025Article
- Three-dimensional optically cleared tissue imaging for analyzing endoscopic images of gastrointestinal neoplasms (with video).Digestive endoscopy : official journal of the Japan Gastroenterological Endoscopy Society · 2025Article
- Pedunculopontine-stimulation obstructs hippocampal theta rhythm and halts movement.Scientific reports · 2025Article
- A cost- and time-efficient method for high-throughput cryoprocessing and tissue analysis using multiplexed tissue molds.Cell reports methods · 2025Article
- Article
- From morphology to single-cell molecules: high-resolution 3D histology in biomedicine.Molecular cancer · 2025Review
- Assembly of glioblastoma tumoroids and cerebral organoids: a 3D in vitro model for tumor cell invasion.Molecular oncology · 2025Article
- Deep imaging of LepRBone research · 2025Article
- Three-dimensional exploration of the chicken embryo, a comparative study of light sheet and histological visualisation.PloS one · 2025Article
- Rapid and efficient optical tissue clearing for volumetric imaging of the intact and injured spinal cord in mice.Frontiers in neuroscience · 2025Article
- 3D visualization of uterus and ovary: tissue clearing techniques and biomedical applications.Frontiers in bioengineering and biotechnology · 2025Review
- 3D visualization of human colon tissue using a modified CUBIC-based tissue-clearing technique.Journal of biological methods · 2025Article
- A Simplified and Robust Immunofluorescence Labeling Method for Complex 3D Cell Cultures: Minimizing Manipulation and Maximizing Data in Whole-Mount Analysis of Organoids, Spheroids, and Co-culture Models.Methods in molecular biology (Clifton, N.J.) · 2025Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
Turbidity and opaqueness are inherent properties of tissues that limit the capacity to acquire microscopic images through large tissues. Creating a uniform refractive index, known as tissue clearing, overcomes most of these issues. These methods have enabled researchers to image large and complex 3D structures with unprecedented depth and resolution. However, tissue clearing has been adopted to a limited extent due to a combination of cost, time, complexity of existing methods and potential negative impact on fluorescence signal. Here, we describe 2Eci (2nd generation ethyl cinnamate-based clearing), which can be used to clear a wide range of tissues in several species, including human organoids,
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.