ArticleDevelopmental biology2019
Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres.
Article in Developmental biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Embryonic lineage-specific iPSC-derived mesenchymal stem/stromal cells exhibit different morphologies and intrinsic functions.iScience · 2026Article
- Article
- The beneficial effects of chick embryo extract preconditioning on hair follicle stem cells: A promising strategy to generate Schwann cells.Cell proliferation · 2023Article
- Wnt/BMP Mediated Metabolic Reprogramming Preserves Multipotency of Neural Crest-Like Stem Cells.Stem cells (Dayton, Ohio) · 2023Article
- Time to go: neural crest cell epithelial-to-mesenchymal transition.Development (Cambridge, England) · 2022Review
- Implantation of a nerve protector embedded with human GMSC-derived Schwann-like cells accelerates regeneration of crush-injured rat sciatic nerves.Stem cell research & therapy · 2022Article
- Harnessing 3D collagen hydrogel-directed conversion of human GMSCs into SCP-like cells to generate functionalized nerve conduits.NPJ Regenerative medicine · 2021Article
- Neural crest-like stem cells for tissue regeneration.Stem cells translational medicine · 2021Review
- Hypoxia inducible factor-2α importance for migration, proliferation, and self-renewal of trunk neural crest cells.Developmental dynamics : an official publication of the American Association of Anatomists · 2021Article
- On the road again: Establishment and maintenance of stemness in the neural crest from embryo to adulthood.Stem cells (Dayton, Ohio) · 2021Review
- In Vitro Maintenance of Multipotent Neural Crest Stem Cells as Crestospheres.Methods in molecular biology (Clifton, N.J.) · 2019Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Neural crest cells have broad migratory and differentiative ability that differs according to their axial level of origin. However, their transient nature has limited understanding of their stem cell and self-renewal properties. While an in vitro culture method has made it possible to maintain cranial neural crest cells as self-renewing multipotent crestospheres (Kerosuo et al., 2015), these same conditions failed to preserve trunk neural crest in a stem-like state. Here we optimize culture conditions for maintenance of avian trunk crestospheres, comprised of both neural crest stem and progenitor cells. Our trunk-derived crestospheres are multipotent and display self-renewal capacity over several weeks. Trunk crestospheres display elevated expression of neural crest cell markers as compared to those characteristic of ventrolateral neural tube or mesodermal fates. Moreover, trunk crestospheres express increased levels of trunk neural crest-enriched markers as compared to cranial crestospheres. Finally, we use lentiviral transduction as a tool to manipulate gene expression in trunk crestospheres. Taken together, this method enables long-term in vitro maintenance and manipulation of multipotent trunk neural crest cells in a premigratory stem or early progenitor state. Trunk crestospheres are a valuable resource for probing mechanisms underlying neural crest stemness and lineage decisions as well as accompanying diseases.
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