Evidence map›Paper›PMID 30664358›Full record

ArticleExperimental biology and medicine (Maywood, N.J.)2019

In vivo miRNA delivery in whitefish: Synthetic MiR92b-3p uptake and the efficacy of gene expression silencing.

Paweł Brzuzan, Maciej Woźny, Bogdan Lewczuk, Maciej Florczyk, Piotr Gomułka, Paulina Budzińska, Michał Wesołowski, Stefan Dobosz

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental biology and medicine (Maywood, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 61% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Paweł Brzuzan1 Department of Environmental Biotechnology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Olsztyn 10-709, Poland.
Maciej Woźny1 Department of Environmental Biotechnology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Olsztyn 10-709, Poland.ORCID 0000-0001-5201-2925
Bogdan Lewczuk2 Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn 10-713, Poland.
Maciej Florczyk1 Department of Environmental Biotechnology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Olsztyn 10-709, Poland.ORCID 0000-0003-2277-312X
Piotr Gomułka3 Department of Ichthyology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Olsztyn 10-719, Poland.
Paulina Budzińska1 Department of Environmental Biotechnology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Olsztyn 10-709, Poland.
Michał Wesołowski1 Department of Environmental Biotechnology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Olsztyn 10-709, Poland.
Stefan Dobosz4 Department of the Salmonid Research in Rutki, Inland Fisheries Institute in Olsztyn, Żukowo 83-330, Poland.
University of Warmia and Mazury in Olsztyn · PLStanisław Sakowicz Inland Fisheries Institute · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

impact statementThe delivery of short snippets of RNA, such as synthetic miRNA agents, is an essential step for achieving RNA-mediated knockdown, which has not been studied in sufficient detail in fish. Our results indicate that a MiR92b-3p mimic may be effectively delivered via intraperitoneal injection to the spleen and the liver of whitefish, and that it likely achieves functionality without causing any apparent toxic effects in the challenged animals. We report the novel finding that the MiR92b-3p mimic reduced the in vivo liver mRNA expression levels of its putative pro-apoptotic targets (p53, cdkn1a, and pcna), and important metabolic genes, e.g. cdo1. This shows that this methodology of MiR92b-3p mimic transfection in vivo may be a useful tool for studies that investigate the molecular pathways that confer pro-proliferative and anti-apoptotic phenotypes or those that regulate intracellular metabolism in fish and other vertebrates.

Indexed as

Gene SilencingAnimalsGene Knockdown TechniquesLiverMicroRNAsPlasmaSalmonidaeTransfectionMicroRNAscdo1imagingoligonucleotides mimicking miRNAsRNA-Seqscreeningtransfection

Identifiers

PMID30664358
PMCPMC6362533
OpenAlexW2913645709

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.