ArticleMolecular medicine reports2019
Mechanical force promotes the proliferation and extracellular matrix synthesis of human gingival fibroblasts cultured on 3D PLGA scaffolds via TGF‑β expression.
Article in Molecular medicine reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.
- MMPs and TIMPs Expression Levels in the Periodontal Ligament during Orthodontic Tooth Movement: A Systematic Review of In Vitro and In Vivo Studies.International journal of molecular sciences · 2021Pooled it
- Vape flavoring chemicals and oral fibroblast viability.Anatomy & cell biology · 2026Review
- Integrative multi-omics and causal inference reveal periodontal ligament cell-macrophage crosstalk under orthodontic force.Progress in orthodontics · 2025Article
- Mechanotransduction-enhanced bioconstructs fabricated using a bioink comprising collagen and omega-3 fatty acids for gingival tissue regeneration.Theranostics · 2025Article
- Hyperbaric Oxygen Therapy to Minimize Orthodontic Relapse in Rabbits.European journal of dentistry · 2024Article
- Pathological mechanisms of cold and mechanical stress in modulating cancer progression.Human cell · 2024Review
- Transcriptome analysis of human peri-implant soft tissue and periodontal gingiva: a paired design study.Clinical oral investigations · 2023Article
- Dynamic Stimulations with Bioengineered Extracellular Matrix-Mimicking Hydrogels for Mechano Cell Reprogramming and Therapy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Local Concentrations of TGF-β1 and IGF-1 Appear Determinant in Regulating Bone Regeneration in Human Postextraction Tooth Sockets.International journal of molecular sciences · 2023Article
- Microbial Poly(hydroxybutyrate-co-hydroxyvalerate) Scaffold for Periodontal Tissue Engineering.Polymers · 2023Article
- Microtubule Assists Actomyosin to Regulate Cell Nuclear Mechanics and Chromatin Accessibility.Research (Washington, D.C.) · 2023Article
- Article
- Intermittent compressive force induces cell cycling and reduces apoptosis in embryoid bodies of mouse induced pluripotent stem cells.International journal of oral science · 2022Article
- Biomechanical and biological responses of periodontium in orthodontic tooth movement: up-date in a new decade.International journal of oral science · 2021Review
- Hyaluronic acidAnnals of translational medicine · 2021Article
- Effects of Smad4 on the expression of caspase‑3 and Bcl‑2 in human gingival fibroblasts cultured on 3D PLGA scaffolds induced by compressive force.International journal of molecular medicine · 2021Article
- Current Trends in In Vitro Modeling to Mimic Cellular Crosstalk in Periodontal Tissue.Advanced healthcare materials · 2021Review
- Co-implantation of magnesium and zinc ions into titanium regulates the behaviors of human gingival fibroblasts.Bioactive materials · 2021Article
- Potential Role of Integrin α₅β₁/Focal Adhesion Kinase (FAK) and Actin Cytoskeleton in the Mechanotransduction and Response of Human Gingival Fibroblasts Cultured on a 3-Dimension Lactide-Co-Glycolide (3D PLGA) Scaffold.Medical science monitor : international medical journal of experimental and clinical research · 2020Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human gingival fibroblasts (HGFs) are responsible for connective tissue repair and scarring, and are exposed to mechanical forces under physiological and pathological conditions. The exact mechanisms underlying gingival tissue reconstruction under mechanical forces remain unclear. The present study aimfed to investigate the effects of mechanical forces on the proliferation and extracellular matrix synthesis in HGFs by establishing a 3‑dimensional (3D) HGF culture model using poly(lactide‑co‑glycolide) (PLGA) scaffolds. HGFs were cultured in 3D PLGA scaffolds and a mechanical force of 0, 5, 15, 25 or 35 g/cm2 was applied to HGFs for 24 h. A mechanical force of 25 g/cm2 induced the highest proliferation rate, and thus was selected for subsequent experiments. Cell viability was determined using the MTT assay at 0, 24, 48 and 72 h. The expression levels of type I collagen (COL‑1) and matrix metallopeptidase (MMP)‑1 were examined by reverse transcription‑quantitative polymerase chain reaction and ELISA, and transforming growth factor (TGF)‑β expression was evaluated by ELISA. The application of mechanical force on HGFs cultured on the 3D PLGA scaffolds resulted in a significant increase in cell proliferation and COL‑1 expression, as well as a decrease in MMP‑1 expression. A TGF‑β1 inhibitor was also applied, which attenuated the effects of mechanical force on HGF proliferation, and COL‑1 and MMP‑1 expression, thus suggesting that TGF‑β signaling pathways may mediate the mechanical force‑induced alterations observed in HGFs. In conclusion, these findings helped to clarify the mechanisms underlying mechanical force‑induced HGF proliferation and ECM synthesis, which may promote the development of targeted therapeutics to treat various diseases, including gingival atrophy caused by orthodontic treatment.
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