ArticleParasite epidemiology and control2019
Development of a method to extract protozoan DNA from black soil.
Article in Parasite epidemiology and control, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Development of optimized DNA extraction protocols for the quantification of Eimeria spp. from chicken feces using real-time PCR.Poultry science · 2026Article
- Isolation and habitat analysis of Balamuthia mandrillaris from soil.Parasitology research · 2024Article
- The Requirement of Genetic Diagnostic Technologies for Environmental Surveillance of Antimicrobial Resistance.Sensors (Basel, Switzerland) · 2021Article
- Article
- Improved Protocol for DNA Extraction from Subsoils Using Phosphate Lysis Buffer.Microorganisms · 2020Article
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesMicroorganisms in environmental samples are identified by sequential screening, isolation, and culture steps, followed by the verification of physiological characteristics and morphological classification. Isolation and purification of Amoebae from soil samples is extremely complex, laborious, and time-consuming and require considerable expertise for morphological evaluation. PCR testing of soil DNA seems to be an effective means for protozoa habitat screening. In this study, we added
methodsSoil allophane is a known DNA adsorbing substance that inhibits the PCR reaction. After comparing the soil properties and allophane contents of 7 soil samples, we attempted to combine multiple cell disruption and DNA purification methods to design an optimal soil DNA extraction method that can be used for downstream PCR analysis.
resultsWe compared five different crushing/refining methods. Amplification of the gene was confirmed by
conclusionThe soil DNA extraction method following protocol V allows DNA amplification of protozoa, including Amoeba, which is difficult to cultivate, thus simplifying the investigation of protozoa habitats and genetic analyses.
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Registered trials
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