ArticleAmerican journal of translational research2018
ROCK inhibitor Y-27632 maintains the propagation and characteristics of hair follicle stem cells.
Article in American journal of translational research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 12 citations in OpenAlex.
- Hair Follicle Organoids Using Human iPSC-Derived Ectodermal Precursor Cells for Hair Regenerative Medicine.ACS biomaterials science & engineering · 2026Article
- Expansion Culture of Hair Follicle Stem Cells through Uniform Aggregation in Microwell Array Devices.ACS biomaterials science & engineering · 2023Article
- Directed differentiation of human pluripotent stem cells into epidermal stem and progenitor cells.Molecular biology reports · 2021Article
- Nanoscale microenvironment engineering for expanding human hair follicle stem cell and revealing their plasticity.Journal of nanobiotechnology · 2021Article
- Hair follicle germs containing vascular endothelial cells for hair regenerative medicine.Scientific reports · 2021Article
- Establishment of an Efficient Primary Culture System for Human Hair Follicle Stem Cells Using the Rho-Associated Protein Kinase Inhibitor Y-27632.Frontiers in cell and developmental biology · 2021Article
- Nanoscale microenvironment engineering based on layer-by-layer self-assembly to regulate hair follicle stem cell fate for regenerative medicine.Theranostics · 2020Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hair follicle stem cells (HFSCs) are an important source for skin tissue engineering studies and clinical applications. Here, we describe a differential enrichment approach to derive HFSCs from hair follicles of vibrissae and ear skin using the Rho-associated protein kinase (ROCK) inhibitor Y-27632. In the presence of Y-27632, primary cultured hair follicle cells grew in clustered colonies surrounded by keratinocyte-like cells and simultaneously expressed three HFSC markers: CD34, K15, and ITGB1. HFSCs cultured in medium containing Y-27632 were presented at a stable ratio of 30.7%, 34.1%, and 32.9% after passages 5, 10, and 15, respectively. By contrast, in medium containing epidermal growth factor, clustered HFSC colonies disappeared after 6 passages and lacked HFSC marker expression. After withdrawal of Y-27632 from the medium, HFSCs rapidly differentiated into keratinocyte-like cells. Furthermore, HFSCs derived with Y-27632 formed spherical clusters in collagen matrix
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30662619PMC6291721W2984179331What OpenQuestion holds
Registered trials
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