ArticleMicrobial ecology2019
High-Throughput Sequencing of the 16S rRNA Gene as a Survey to Analyze the Microbiomes of Free-Living Ciliates Paramecium.
Article in Microbial ecology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 20 citations in OpenAlex.
- Culture-Independent Single-Cell PacBio Sequencing Reveals EpibioticMicroorganisms · 2023Article
- Changes in microbial community during hydrolyzed sludge reduction.Frontiers in microbiology · 2023Article
- Establishment of an unfed strain ofFrontiers in microbiology · 2023Article
- Editorial: Microbial associations formed and hosted by protists, algae, and fungi.Frontiers in microbiology · 2023Article
- Among-Strain Variation in Resistance ofFrontiers in microbiology · 2020Article
- The Obligate Symbiont "Frontiers in microbiology · 2020Article
- Epidemiology of Nucleus-Dwelling Holospora: Infection, Transmission, Adaptation, and Interaction with Paramecium.Results and problems in cell differentiation · 2020Review
- A single-cell genome perspective on studying intracellular associations in unicellular eukaryotes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- The core microbiome of sessile ciliate Stentor coeruleus is not shaped by the environment.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Ciliates are the largest group of ubiquitous aquatic bacterivorous protists, and many species are easily cultivated. However, only few studies reported prokaryotic communities naturally associated with ciliate cells. Herein, we analyzed the microbiome composition of several strains of Paramecium (Ciliophora) originating from different locations and belonging to two morpho-species by high-throughput sequencing (HTS) of the 16S rRNA gene. Possible reasons of HTS results bias were addressed comparing DNA libraries obtained using different primers and different number of ciliate cells. Microbiomes associated with ciliates and their environments were always significantly different by prokaryotic taxonomic composition and bacterial richness. There were also pronounced differences between Paramecium strains. Interestingly, potentially pathogenic bacteria were revealed in Paramecium microbiomes.
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Registered trials
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