Evidence map›Paper›PMID 30661082›Full record

Trial reportPediatric research2019

Dried blood spot compared to plasma measurements of blood-based biomarkers of brain injury in neonatal encephalopathy.

An N Massaro, Yvonne W Wu, Theo K Bammler, James W MacDonald, Amit Mathur, Taeun Chang, Dennis Mayock, Sarah B Mulkey, Krisa van Meurs, Zahra Afsharinejad and 1 more

Registry-linked trialOpen access · bronzeAbstract readClinical Trial, Phase IIComparative StudyMulticenter Study
PubMed Publisher
In one paragraph

Trial report in Pediatric research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01913340 (Neonatal Erythropoietin And Therapeutic Hypothermia Outcomes Study), which is not on this map. Cited by 11 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 1 pooled it
2.9field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT01913340 phase1 / phase2completednot on this map

Neonatal Erythropoietin And Therapeutic Hypothermia Outcomes Study

TypeinterventionalSponsorUniversity of California, San FranciscoRan2013 to 2016Enrolled50ConditionsHypoxic-ischemic Encephalopathy, Neonatal Encephalopathy, Birth AsphyxiaArmsErythropoietin, Normal saline
3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 1 synthesis or guideline pooled it, 14 citations in OpenAlex.

  1. Pooled it
  2. Observational
  3. Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Review
  10. Article
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 1 country.

An N MassaroPediatrics - Division of Neonatology, Children's National Health Systems and The George Washington University School of Medicine, Washington, DC, USA. anguyenm@cnmc.org.
Yvonne W WuNeurology and Pediatrics, UCSF, San Francisco, CA, USA.
Theo K BammlerDepartment of Environmental & Occupational Health Sciences, University of Washington, Seattle, WA, USA.
James W MacDonaldDepartment of Environmental & Occupational Health Sciences, University of Washington, Seattle, WA, USA.
Amit MathurPediatrics, Washington University School of Medicine, St. Louis, MO, USA.
Taeun ChangNeurology and Pediatrics, Children's National Health Systems and The George Washington University School of Medicine, Washington, DC, USA.
Dennis MayockPediatrics-Division of Neonatology, University of Washington, Seattle, WA, USA.
Sarah B MulkeyNeurology and Pediatrics, Children's National Health Systems and The George Washington University School of Medicine, Washington, DC, USA.
Krisa van MeursPediatrics, Stanford, Palo Alto, CA, USA.
Zahra AfsharinejadDepartment of Environmental & Occupational Health Sciences, University of Washington, Seattle, WA, USA.
Sandra E JuulPediatrics-Division of Neonatology, University of Washington, Seattle, WA, USA.
University of Washington · USGeorge Washington University · USChildren's National · USUniversity of California, San Francisco · USWashington University in St. Louis · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundData correlating dried blood spots (DBS) and plasma concentrations for neonatal biomarkers of brain injury are lacking. We hypothesized that candidate biomarker levels determined from DBS can serve as a reliable surrogate for plasma levels.

methodsIn the context of a phase II multi-center trial evaluating erythropoietin for neuroprotection in neonatal encephalopathy (NE), DBS were collected at enrollment ( < 24 h), day 2, 4, and 5. Plasma was collected with the first and last DBS. The relationship between paired DBS-plasma determinations of brain-specific proteins and cytokines was assessed by correlation and Bland-Altman analyses. For analytes with consistent DBS-plasma associations, DBS-derived biomarker levels were related to brain injury by MRI and 1-year outcomes.

resultsWe enrolled 50 newborns with NE. While S100B protein, tumor necrosis factor α, interleukin (IL)1 β, IL-6, IL-8 demonstrated significant DBS-plasma correlations, Bland-Altman plots demonstrated that the methods are not interchangeable, with a 2 to 4-fold error between measurements. No significant relationships were found between DBS levels of TNFα, IL-6, and IL-8 and outcomes.

conclusionFurther work is needed to optimize elution and assay methods before using DBS specimens as a reliable surrogate for plasma levels of candidate brain injury biomarkers in NE.

Indexed as

Dried Blood Spot TestingBiomarkersBrainBrain InjuriesErythropoietinFemaleFollow-Up StudiesHumansInfantInfant, NewbornInfant, Newborn, DiseasesMagnetic Resonance ImagingMaleNeuroprotectionProspective StudiesTreatment OutcomeBiomarkersEPO protein, humanErythropoietin

Identifiers

PMID30661082
OpenAlexW2909703922

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.