ArticleChemistry and physics of lipids2019
Lipidomic consequences of phospholipid synthesis defects in Escherichia coli revealed by HILIC-ion mobility-mass spectrometry.
Article in Chemistry and physics of lipids, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 38 citations in OpenAlex.
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- Advanced Method for the In Vivo Measurements of Lysophospholipid Translocation Across the Inner (Cytoplasmic) Membrane of Escherichia coli.Methods in molecular biology (Clifton, N.J.) · 2025Article
- From big data to big insights: statistical and bioinformatic approaches for exploring the lipidome.Analytical and bioanalytical chemistry · 2024Review
- Do Nanodisc Assembly Conditions Affect Natural Lipid Uptake?Journal of the American Society for Mass Spectrometry · 2023Article
- Collision cross section measurement and prediction methods in omics.Journal of mass spectrometry : JMS · 2023Review
- A sustainable and regioselective synthesis of Hemi-bis(monoacylglycero)phosphates and bis(diacylglycero)phosphates.iScience · 2023Article
- A rapid single-phase extraction for polar staphylococcal lipids.Analytical and bioanalytical chemistry · 2023Article
- Construction of a Bacterial Lipidomics Analytical Platform: Pilot Validation with Bovine Paratuberculosis Serum.Metabolites · 2023Article
- Strain-Level Discrimination of Bacteria by Liquid Chromatography and Paper Spray Ion Mobility Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2023Article
- Transient Complexity ofMetabolites · 2022Article
- Temporal changes in the brain lipidome during neurodevelopment of Smith-Lemli-Opitz syndrome mice.The Analyst · 2022Article
- Recent advances in the mass spectrometric profiling of bacterial lipids.Current opinion in chemical biology · 2021Review
- HutW fromBiochemistry · 2021Article
- A Novel Approach to Characterize the Lipidome of Marine ArchaeonFrontiers in microbiology · 2021Article
- Untargeted Lipidomics Analysis of the CyanobacteriumInternational journal of molecular sciences · 2020Article
- LiPydomics: A Python Package for Comprehensive Prediction of Lipid Collision Cross Sections and Retention Times and Analysis of Ion Mobility-Mass Spectrometry-Based Lipidomics Data.Analytical chemistry · 2020Article
- Cationic π-Conjugated Polyelectrolyte Shows Antimicrobial Activity by Causing Lipid Loss and Lowering Elastic Modulus of Bacteria.ACS applied materials & interfaces · 2020Article
- Recent applications of mass spectrometry in bacterial lipidomics.Analytical and bioanalytical chemistry · 2020Review
- Lipidomic Analysis of Roseobacters of the Pelagic RCA Cluster and Their Response to Phosphorus Limitation.Frontiers in microbiology · 2020Article
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2 authors at 1 institution in 1 country.
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Abstract
Our understanding of phospholipid biosynthesis in Gram-positive and Gram-negative bacteria is derived from the prototypical Gram-negative organism Escherichia coli. The inner and outer membranes of E. coli are largely composed of phosphatidylethanolamine (PE), minor amounts of phosphatidylglycerol (PG) and cardiolipin (CL). We report here the utility of hydrophilic interaction liquid chromatography (HILIC) paired with ion mobility-mass spectrometry (IM-MS) for the comprehensive analysis of the E. coli lipidome. Using strains with chromosomal deletions in the PG and CL synthesis genes pgsA and clsABC, respectively, we show that defective phospholipid biosynthesis in E. coli results in fatty-acid specific changes in select lipid classes and the presence of the minor triacylated phospholipids, acylphosphatidyl glycerol (acylPG) and N-acylphosphatidylethanolamine (N-acylPE). Notably, acylPGs were accumulated in the clsABC-KO strain, but were absent in other mutant strains. The separation of 1-lyso and 2-lyso-phosphatidylethanolamines (lysoPEs) is demonstrated in both the HILIC and IM dimensions. Using our previously validated calibration method, collision cross section values of nearly 200 phospholipids found in E. coli were determined on a traveling wave IM-MS platform, including newly reported values for cardiolipins, positional isomers of lysoPEs, acylPGs and N-acylPEs.
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