ArticleBiochimica et biophysica acta. Biomembranes2019
Single-channel permeability and glycerol affinity of human aquaglyceroporin AQP3.
Article in Biochimica et biophysica acta. Biomembranes, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Article
- Application of the Transition State Theory in the Study of the Osmotic Permeabilities of AQP7, AQP10 and GlpF.Membranes · 2025Article
- CBL-mediated AQP1 ubiquitination aggravates kidney-yang deficiency syndrome by promoting lipid metabolism dysregulation.European journal of medical research · 2025Article
- Narrowed pore conformations of aquaglyceroporins AQP3 and GlpF.Nature communications · 2025Article
- Role of aquaporins in corneal healing post chemical injury.Experimental eye research · 2023Review
- Methods for studying mammalian aquaporin biology.Biology methods & protocols · 2023Review
- Targeting Aquaporin-3 Attenuates Skin Inflammation in Rosacea.International journal of biological sciences · 2023Article
- A potential link between AQP3 and SLC14A1 gene expression level and clinical parameters of maintenance hemodialysis patients.BMC nephrology · 2022Article
- Aquaglyceroporin AQP7's affinity for its substrate glycerol: Have we reached convergence in the computed values of glycerol-aquaglyceroporin affinity?RSC advances · 2022Article
- Effect of cryoprotectant concentration on bovine oocyte permeability and comparison of two membrane permeability modelling approaches.Scientific reports · 2021Article
- Adverse effects of LPS on membrane proteins in lactating bovine mammary epithelial cells.Cell and tissue research · 2021Article
- AQP3 Facilitates Proliferation and Adipogenic Differentiation of Porcine Intramuscular Adipocytes.Genes · 2020Article
- Effects of Betaine Supplementation on Live Performance, Selected Blood Parameters, and Expression of Water Channel and Stress-Related mRNA Transcripts of Delayed Placement Broiler Chicks.Frontiers in veterinary science · 2020Article
- Quantitative study of unsaturated transport of glycerol through aquaglyceroporin that has high affinity for glycerol.RSC advances · 2020Article
- Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
For its fundamental relevance, transport of water and glycerol across the erythrocyte membrane has long been investigated before and after the discovery of aquaporins (AQPs), the membrane proteins responsible for water and glycerol transport. AQP1 is abundantly expressed in the human erythrocyte for maintaining its hydrohomeostasis where AQP3 is also expressed (at a level ~30-folds lower than AQP1) facilitating glycerol transport. This research is focused on two of the remaining questions: How permeable is AQP3 to water? What is the glycerol-AQP3 affinity under near-physiological conditions? Through atomistic modelling and large-scale simulations, we found that AQP3 is two to three times more permeable to water than AQP1 and that the glycerol-AQP3 affinity is approximately 500/M. Using these computed values along with the data from the latest literature on AQP1 and on erythrocyte proteomics, we estimated the water and glycerol transport rates across the membrane of an entire erythrocyte. We used these rates to predict the time courses of erythrocyte swelling-shrinking in response to inward and outward osmotic gradients. Experimentally, we monitored the time course of human erythrocytes when subject to an osmotic or glycerol gradient with light scattering in a stopped-flow spectrometer. We observed close agreement between the experimentally measured and the computationally predicted time courses of erythrocytes, which corroborated our computational conclusions on the AQP3 water-permeability and the glycerol-AQP3 affinity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.