ArticleNeural development2019
An ancient role for collier/Olf/Ebf (COE)-type transcription factors in axial motor neuron development.
Article in Neural development, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 30 citations in OpenAlex.
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- Postnatal expression of the transcription factor Ebf2 in motivation, reward, and pain-related circuits of the mouse brain.Frontiers in neuroanatomy · 2026Article
- Regulation of motor neuron differentiation in thebioRxiv : the preprint server for biology · 2025Article
- Neuron-specific Agrin splicing by Nova RNA-binding proteins regulates conserved neuromuscular junction development in chordates.PLoS biology · 2025Article
- Molecular and Cellular Mechanisms of Motor Circuit Development.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Review
- A conserved RNA switch for acetylcholine receptor clustering at neuromuscular junctions in chordates.bioRxiv : the preprint server for biology · 2024Article
- Maintenance of neuronal identity in C. elegans and beyond: Lessons from transcription and chromatin factors.Seminars in cell & developmental biology · 2024Review
- DNA Conserved in Diverse Animals Since the Precambrian Controls Genes for Embryonic Development.Molecular biology and evolution · 2023Article
- Prenatal benzene exposure in mice alters offspring hypothalamic development predisposing to metabolic disease in later life.Chemosphere · 2023Article
- Single-cell transcriptomic analysis reveals diversity within mammalian spinal motor neurons.Nature communications · 2023Article
- Transcriptional dynamics of murine motor neuron maturation in vivo and in vitro.Nature communications · 2022Article
- Article
- The histone demethylase Kdm6b regulates subtype diversification of mouse spinal motor neurons during development.Nature communications · 2022Article
- Establishing the Molecular and Functional Diversity of Spinal Motoneurons.Advances in neurobiology · 2022Article
- A cis-regulatory change underlying the motor neuron-specific loss of Ebf expression in immotile tunicate larvae.Evolution & development · 2021Article
- Ebf Activates Expression of a Cholinergic Locus in a Multipolar Motor Ganglion Interneuron Subtype inFrontiers in neuroscience · 2021Article
- Article
- Dynamic Expression and New Functions of Early B Cell Factor 2 in Cerebellar Development.Cerebellum (London, England) · 2019Article
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7 authors at 4 institutions in 2 countries.
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Abstract
backgroundMammalian motor circuits display remarkable cellular diversity with hundreds of motor neuron (MN) subtypes innervating hundreds of different muscles. Extensive research on limb muscle-innervating MNs has begun to elucidate the genetic programs that control animal locomotion. In striking contrast, the molecular mechanisms underlying the development of axial muscle-innervating MNs, which control breathing and spinal alignment, are poorly studied.
methodsOur previous studies indicated that the function of the Collier/Olf/Ebf (COE) family of transcription factors (TFs) in axial MN development may be conserved from nematodes to simple chordates. Here, we examine the expression pattern of all four mouse COE family members (mEbf1-mEbf4) in spinal MNs and employ genetic approaches in both nematodes and mice to investigate their function in axial MN development.
resultsWe report that mEbf1 and mEbf2 are expressed in distinct MN clusters (termed "columns") that innervate different axial muscles. Mouse Ebf1 is expressed in MNs of the hypaxial motor column (HMC), which is necessary for breathing, while mEbf2 is expressed in MNs of the medial motor column (MMC) that control spinal alignment. Our characterization of Ebf2 knock-out mice uncovered a requirement for Ebf2 in the differentiation program of a subset of MMC MNs and revealed for the first time molecular diversity within MMC neurons. Intriguingly, transgenic expression of mEbf1 or mEbf2 can rescue axial MN differentiation and locomotory defects in nematodes (Caenorhabditis elegans) lacking unc-3, the sole C. elegans ortholog of the COE family, suggesting functional conservation among mEbf1, mEbf2 and nematode UNC-3.
conclusionsThese findings support the hypothesis that genetic programs controlling axial MN development are deeply conserved across species, and further advance our understanding of such programs by revealing an essential role for Ebf2 in mouse axial MNs. Because human mutations in COE orthologs lead to neurodevelopmental disorders characterized by motor developmental delay, our findings may advance our understanding of these human conditions.
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