ArticleBMC research notes2019
Simultaneous extraction of mRNA and microRNA from whole blood stabilized in tempus tubes.
Article in BMC research notes, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Whole-blood transcriptomic response to whole-body hyperthermia in participants with major depressive disorder.Brain, behavior, & immunity - health · 2026Article
- The Use of a Novel Container for Secured Transport and Storage of Biological Material for Quantitative Human RNA Analysis.International journal of molecular sciences · 2024Article
- Characteristics of RNA Stabilizer RNApro for Peripheral Blood Collection.Diagnostics (Basel, Switzerland) · 2024Article
- Technical assessment of different extraction methods and transcriptome profiling of RNA isolated from small volumes of blood.Scientific reports · 2023Article
- Whole blood RNA extraction efficiency contributes to variability in RNA sequencing data sets.PloS one · 2023Article
- Optimized protocol for the extraction of RNA and DNA from frozen whole blood sample stored in a single EDTA tube.Scientific reports · 2021Article
- Evaluation of RNA purification methods by using different blood stabilization tubes: identification of key features for epidemiological studies.BMC research notes · 2020Article
- An Analytically and Diagnostically Sensitive RNA Extraction and RT-qPCR Protocol for Peripheral Blood Mononuclear Cells.Frontiers in immunology · 2020Article
- Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveStudies of mRNA and miRNA expression profiling increasingly use stabilized whole blood. Commercial RNA extraction kits do not provide information about the simultaneous recovery of both mRNA and miRNA. This study evaluated yield, quality, integrity and representation of mRNA and miRNA from whole blood stabilized in Tempus tubes using three RNA extraction kits; two filter-based (Tempus and Norgen) and one bead-based (MagMax; manual vs. semi-automated, and with and without DNase treatment).
resultsAll RNA extraction kits and methods resulted in similar yields of mRNA (total RNA yield, quality, integrity and representation) whereas there were differences in yields of miRNA. MagMax, either manual or semi-automated, with or without DNase treatment, yielded 1.6-2.2-fold more miRNA than Tempus and Norgen kits. In addition, MagMax and Norgen methods gave greater than 12-fold more and 3.3-fold less enrichment of specific miRNA targets, respectively, in comparison to Tempus extraction reagents. This study identified MagMax kit for simultaneous recovery of both mRNA and miRNA from whole blood collected in Tempus tubes.
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Registered trials
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