Evidence map›Paper›PMID 30658577›Full record

ArticleBMC genomics2019

High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers.

Charlotte Gustafsson, Ayla De Paepe, Christian Schmidl, Robert Månsson

Abstract read
In one paragraph

Article in BMC genomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed.

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  12. AP-1 is a temporally regulated dual gatekeeper of reprogramming to pluripotency.Proceedings of the National Academy of Sciences of the United States of America · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Charlotte GustafssonCenter for Hematology and Regenerative Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden.
Ayla De PaepeCenter for Hematology and Regenerative Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden.
Christian SchmidlRegensburg Centre for Interventional Immunology (RCI) and University Medical Center, Regensburg, Germany.
Robert MånssonCenter for Hematology and Regenerative Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden. robert.mansson@ki.se.ORCID http://orcid.org/0000-0003-0738-0328

Funding

Cancerfonden 160667Knut och Alice Wallenbergs Stiftelse KAW 2014.0249Stiftelsen för Strategisk Forskning FFL12-0240Vetenskapsrådet (SE) 2015-03485_3
6 · The paper itself

Abstract

backgroundChromatin immunoprecipitation coupled to sequencing (ChIP-seq) is widely used to map histone modifications and transcription factor binding on a genome-wide level.

resultsWe present high-throughput ChIPmentation (HT-ChIPmentation) that eliminates the need for DNA purification prior to library amplification and reduces reverse-crosslinking time from hours to minutes.

conclusionsThe resulting workflow is easily established, extremely rapid, and compatible with requirements for very low numbers of FACS sorted cells, high-throughput applications and single day data generation.

Indexed as

Gene LibraryProtein Processing, Post-TranslationalCell CountCell Line, TumorChromatin ImmunoprecipitationDNAHigh-Throughput Nucleotide SequencingHistone CodeHumansProtein BindingDNAChIPmentationChIP-seqChromatin immunoprecipitationEpigeneticsHigh-throughput genomics

Identifiers

PMID30658577
PMCPMC6339279

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.