ArticleRadiation research2019
RABiT-II: A Fully-Automated Micronucleus Assay System with Shortened Time to Result.
Article in Radiation research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Multicentered Biospecimen Analyses after 4 Gy Sublethal Total Body Irradiation in Rhesus Macaques.Radiation research · 2025Article
- Centers for Medical Countermeasures against Radiation Consortium: Past, Present, and Beyond.Radiation research · 2025Review
- Potentials of cytokinesis blocked micronucleus assay in radiation triage and biological dosimetry.Journal, genetic engineering & biotechnology · 2024Review
- RABiT-III: an Automated Micronucleus Assay at a Non-Specialized Biodosimetry Facility.Radiation research · 2024Article
- Sex and dose rate effects in automated cytogenetics.Radiation protection dosimetry · 2023Article
- Cross-platform validation of a mouse blood gene signature for quantitative reconstruction of radiation dose.Scientific reports · 2022Article
- MicroRNA-149 suppresses osteogenic differentiation of mesenchymal stem cells via inhibition of AKT1-dependent Twist1 phosphorylation.Cell death discovery · 2022Article
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- Cytogenetic biological dosimetry assays: recent developments and updates.Radiation oncology journal · 2021Article
- Whole blood gene expression within days after total-body irradiation predicts long term survival in Gottingen minipigs.Scientific reports · 2021Article
- Transportation container for pre-processing cytogenetic assays in radiation accidents.Scientific reports · 2021Article
- Gene Expression Profiles from Heart, Lung and Liver Samples of Total-Body-Irradiated Minipigs: Implications for Predicting Radiation-Induced Tissue Toxicity.Radiation research · 2020Article
- The use of a centrifuge-free RABiT-II system for high-throughput micronucleus analysis.Journal of radiation research · 2020Article
- Development of an integrated fingerstick blood self-collection device for radiation countermeasures.PloS one · 2019Article
- Multiparameter imaging flow cytometry-based cytokinesis-block micronucleus assay: Reduction of culture time and blood volume for improved efficiency.Mutation research. Genetic toxicology and environmental mutagenesisArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In this work, we describe a fully automated cytokinesis-block micronucleus (CBMN) assay with a significantly shortened time to result, motivated by the need for rapid high-throughput biodosimetric estimation of radiation doses from small-volume human blood samples. The Rapid Automated Biodosimetry Tool (RABiT-II) currently consists of two commercial automated systems: a PerkinElmer cell::explorer Workstation and a GE Healthcare IN Cell Analyzer 2000 Imager. Blood samples (30 μl) from eight healthy volunteers were gamma-ray irradiated ex vivo with 0 (control), 0.5, 1.5, 2.5, 3.5 or 4.5 Gy and processed with full automation in 96-well plates on the RABiT-II system. The total cell culture time was 54 h and total assay time was 3 days. DAPI-stained fixed samples were imaged on an IN Cell Analyzer 2000 with fully-automated image analysis using the GE Healthcare IN Cell Developer Toolbox version 1.9. A CBMN dose-response calibration curve was established, after which the capability of the system to predict known doses was assessed. Various radiation doses for irradiated samples from two donors were estimated within 20% of the true dose (±0.5 Gy below 2 Gy) in 97% of the samples, with the doses in some 5 Gy irradiated samples being underestimated by up to 25%. In summary, the findings from this work demonstrate that the accelerated CBMN assay can be automated in a high-throughput format, using commercial biotech robotic systems, in 96-well plates, providing a rapid and reliable bioassay for radiation exposure.
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