ArticleMicrobial cell factories2019
Simultaneous enhancement of the beta-exo synergism and exo-exo synergism in Trichoderma reesei cellulase to increase the cellulose degrading capability.
Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 54 citations in OpenAlex.
- Microbial Community Restructuring Enhances Composting Efficiency: Synergistic Roles of Thermal Cycling and Fungal Inoculants (Fomes lignosus and Penicillium glabrum) in Metabolic Adaptation.Microbial biotechnology · 2025Article
- Trichoderma reesei Cellulase in the Monoculture and the Mixed Culture with Aspergillus niger: From Transcripts to Proteins.Applied biochemistry and biotechnology · 2025Article
- Regulation of genes encoding polysaccharide-degrading enzymes in Penicillium.Applied microbiology and biotechnology · 2024Review
- Improved consolidated bioprocessing for itaconic acid production by simultaneous optimization of cellulase and metabolic pathway of Neurospora crassa.Biotechnology for biofuels and bioproducts · 2024Article
- Effective semi-fed-batch saccharification with high lignocellulose loading using co-culture ofFrontiers in microbiology · 2024Article
- Effect of itaconic acid production on Neurospora crassa in consolidated bioprocessing of cellulose.Microbial cell factories · 2023Article
- Consolidated bioprocessing of lignocellulose for production of glucaric acid by an artificial microbial consortium.Biotechnology for biofuels · 2021Article
- Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30.BMC genomics · 2020Article
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4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundCellulase is the one of the largest contributors to the high production costs of the lignocellulose-based biorefineries. As the most widely used cellulase producer, Trichoderma reesei has two weaknesses, deficiencies in β-glucosidase and cellobiohydrolase II. This work aimed at solving this problem by simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase to increase the cellulose degrading capability, i.e. enhanced co-expression of the β-glucosidase gene the cellobiohydrolase II gene of T. reesei.
resultsEnhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 was found successful in overcoming the two weaknesses. Filter paper activities of T. reesei cellulase were greatly elevated, which were 7.21 ± 0.45 (E7, Aabgl1 and Trcbh2) and 7.69 ± 0.42 (F6, Anbgl1 and Trcbh2) FPIU/mL. They were much higher than that of the parental strain Rut-C30, 2.45 ± 0.36 FPIU/mL. Enzymatic hydrolysis yields were also improved, from 67.22 ± 1.61% by Rut-C30 cellulase to 87.98 ± 0.65% by E7 cellulase and 86.50 ± 1.01% by F6 cellulase. The substrate loading for 1 g glucose release from SECS were decreased, from 2.9637 g SECS using Rut-C30 cellulase to 2.0291 g SECS using E7 cellulase and 2.0573 g SECS using F6 cellulase. As a result, the efficiency of the process from SECS to glucose was substantially improved.
conclusionsEnhanced co-expression of the β-glucosidase gene and the cellobiohydrolase II gene in T. reesei using the strong promoter Pcbh1 in T. reesei was proven triumphal in the simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. reesei cellulase. This strategy also improved the cellulase production, enzymatic hydrolysis yield and the efficiency of the process from SECS to glucose in the context of on-site cellulase production. This work is a commendable attempt in the cellulase composition optimization at the transcriptional level.
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