Evidence map›Paper›PMID 30656621›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2019

Chemically Defined Neural Conversion of Human Pluripotent Stem Cells.

Yu Chen, Carlos A Tristan, Sunil K Mallanna, Pinar Ormanoglu, Steven Titus, Anton Simeonov, Ilyas Singeç

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 98% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Yu ChenStem Cell Translation Laboratory (SCTL), Division of Pre-Clinical Innovation, NIH National Center for Advancing Translational Sciences (NCATS), NIH Regenerative Medicine Program, Rockville, MD, USA.
Carlos A TristanStem Cell Translation Laboratory (SCTL), Division of Pre-Clinical Innovation, NIH National Center for Advancing Translational Sciences (NCATS), NIH Regenerative Medicine Program, Rockville, MD, USA.
Sunil K MallannaStem Cell Translation Laboratory (SCTL), Division of Pre-Clinical Innovation, NIH National Center for Advancing Translational Sciences (NCATS), NIH Regenerative Medicine Program, Rockville, MD, USA.
Pinar OrmanogluStem Cell Translation Laboratory (SCTL), Division of Pre-Clinical Innovation, NIH National Center for Advancing Translational Sciences (NCATS), NIH Regenerative Medicine Program, Rockville, MD, USA.
Steven TitusStem Cell Translation Laboratory (SCTL), Division of Pre-Clinical Innovation, NIH National Center for Advancing Translational Sciences (NCATS), NIH Regenerative Medicine Program, Rockville, MD, USA.
Anton SimeonovStem Cell Translation Laboratory (SCTL), Division of Pre-Clinical Innovation, NIH National Center for Advancing Translational Sciences (NCATS), NIH Regenerative Medicine Program, Rockville, MD, USA.
Ilyas SingeçStem Cell Translation Laboratory (SCTL), Division of Pre-Clinical Innovation, NIH National Center for Advancing Translational Sciences (NCATS), NIH Regenerative Medicine Program, Rockville, MD, USA. ilyas.singec@nih.gov.
National Center for Advancing Translational Sciences · US

Funding

Stem Cell Translation Laboratory (SCTL)ZIATR000244 · NCATS · NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES · PI TRISTAN, CARLOS · 2015 to 2024
$58.3M
Small molecule screening programZ01HG200319 · NHGRI · NATIONAL HUMAN GENOME RESEARCH INSTITUTE · PI AUSTIN, CHRISTOPHER P · 2004 to 2008
$32.3M
Intramural NIH HHS Z01 HG200319
6 · The paper itself

Abstract

Human pluripotent stem cells (hPSCs) are characterized by their ability to self-renew and differentiate into any cell type of the human body. To fully utilize the potential of hPSCs for translational research and clinical applications, it is critical to develop rigorous cell differentiation protocols under feeder-free conditions that are efficient, reproducible, and scalable for high-throughput projects. Focusing on neural conversion of hPSCs, here we describe robust small molecule-based procedures that generate neural stem cells (NSCs) in less than a week under chemically defined conditions. These protocols can be used to dissect the mechanisms of neural lineage entry and to further develop systematic protocols that produce the cellular diversity of the central nervous system at industrial scale.

Indexed as

AnimalsBiomarkersCell Culture TechniquesCell DifferentiationEmbryonic Stem CellsHumansImmunohistochemistryImmunophenotypingInduced Pluripotent Stem CellsMiceNeural Stem CellsPluripotent Stem CellsBiomarkersCell differentiationCoating substrateCulture mediumEmbryonic stem cellInduced pluripotent stem cellNeural inductionPathway inhibitionPluripotencySmall molecules

Identifiers

PMID30656621
PMCPMC6908303
OpenAlexW2911114395

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.