ArticleNature communications2019
A minimal biochemical route towards de novo formation of synthetic phospholipid membranes.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Article
- Causal Links Between the Plasma Lipidome and Cognitive Performance: Targets, Pathways, and Lifestyle Influences.Molecular neurobiology · 2026Article
- Image-based phenotypic sorting of synthetic cells.Science advances · 2026Article
- Breaking the membrane heredity paradox through de novo protocell formation.Nature communications · 2026Article
- DNA affects the phenotype of fuel-dependent coacervate droplets.Nature communications · 2026Article
- A synthetic cell with integrated DNA self-replication and lipid biosynthesis.Nature communications · 2026Article
- Construction of Phospholipid-Like Vesicles by Aqueous Aminolysis of Acyl Thioesters With Diamino Acids.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells.FEBS letters · 2025Review
- Synthetic cells by the numbers.iScience · 2025Review
- The Hidden Cost of Fusion: Intrinsic Asymmetry in Vesicle Fusion Restricts Synthetic Cell Growth.bioRxiv : the preprint server for biology · 2025Article
- From experimental clues to theoretical modeling: Evolution associated with the membrane-takeover at an early stage of life.PLoS computational biology · 2025Article
- Pepticombisomes: Biomimetic Vesicles Crafted From Recombinant Supercharged Polypeptides with Uniformly Distributed Side-Chains.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Self-growing protocell models in aqueous two-phase system induced by internal DNA replication reaction.Nature communications · 2025Article
- Synthetic Lipid Biology.Chemical reviews · 2025Review
- Selective peptide bond formation via side chain reactivity and self-assembly of abiotic phosphates.Nature communications · 2025Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Chemically Driven Division of Protocells by Membrane Budding.Journal of the American Chemical Society · 2024Article
- Biosynthesis of isonitrile lipopeptides.Current opinion in chemical biology · 2024Review
- Iterative design of training data to control intricate enzymatic reaction networks.Nature communications · 2024Article
- Rapid Formation of Non-canonical Phospholipid Membranes by Chemoselective Amide-Forming Ligations with Hydroxylamines.Angewandte Chemie (International ed. in English) · 2024Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
All living cells consist of membrane compartments, which are mainly composed of phospholipids. Phospholipid synthesis is catalyzed by membrane-bound enzymes, which themselves require pre-existing membranes for function. Thus, the principle of membrane continuity creates a paradox when considering how the first biochemical membrane-synthesis machinery arose and has hampered efforts to develop simplified pathways for membrane generation in synthetic cells. Here, we develop a high-yielding strategy for de novo formation and growth of phospholipid membranes by repurposing a soluble enzyme FadD10 to form fatty acyl adenylates that react with amine-functionalized lysolipids to form phospholipids. Continuous supply of fresh precursors needed for lipid synthesis enables the growth of vesicles encapsulating FadD10. Using a minimal transcription/translation system, phospholipid vesicles are generated de novo in the presence of DNA encoding FadD10. Our findings suggest that alternate chemistries can produce and maintain synthetic phospholipid membranes and provides a strategy for generating membrane-based materials.
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