ArticleScience (New York, N.Y.)2019
Cortical column and whole-brain imaging with molecular contrast and nanoscale resolution.
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Article in Science (New York, N.Y.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 181 papers.
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181citing papers in PubMed
71.9field-weighted citation impact, top 1% of its field
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181 citing papers in PubMed, 395 citations in OpenAlex.
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- Layer 5 myelination gates corticothalamic coincidence detection.Nature communications · 2025Article
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- Mesoscale volumetric fluorescence imaging at nanoscale resolution by photochemical sectioning.Science (New York, N.Y.) · 2025Article
- Combinatorial protein barcodes enable self-correcting neuron tracing with nanoscale molecular context.bioRxiv : the preprint server for biology · 2025Article
121 more citing papers are in PubMed but not listed here.
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Authors and funding
31 authors at 7 institutions in 1 country.
Ruixuan Gao *MIT Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA.ORCID 0000-0001-7137-2512
Shoh M Asano *MIT Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA.ORCID 0000-0002-9201-1350
Srigokul Upadhyayula *Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.ORCID 0000-0001-6727-5883
Igor PisarevJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.ORCID 0000-0003-4353-4945
Daniel E MilkieJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.ORCID 0000-0002-3917-6965
Tsung-Li LiuJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.ORCID 0000-0001-7965-4725
Ved SinghJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
Austin GravesJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
Grace H HuynhMIT Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA.ORCID 0000-0002-3801-8041
Yongxin ZhaoMIT Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA.ORCID 0000-0003-4188-5725
John BogovicJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.ORCID 0000-0002-4829-9457
Jennifer ColonellJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
Carolyn M OttJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
Christopher Zugatesarivis AG, 1875 Connecticut Avenue NW, 10th floor, Washington, DC 20009, USA.ORCID 0000-0003-1882-3665
Susan TappanMBF Bioscience, 185 Allen Brook Lane, Suite 101, Williston, VT 05495, USA.ORCID 0000-0001-5120-3770
Alfredo RodriguezMBF Bioscience, 185 Allen Brook Lane, Suite 101, Williston, VT 05495, USA.
Kishore R MosaligantiDepartment of Systems Biology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
Shu-Hsien SheuJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.ORCID 0000-0003-0758-4654
H Amalia PasolliJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
Song PangJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
C Shan XuJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.ORCID 0000-0002-8564-7836
Sean G MegasonDepartment of Systems Biology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.ORCID 0000-0002-9330-2934
Harald HessJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.ORCID 0000-0003-3000-1533
Jennifer Lippincott-SchwartzJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.ORCID 0000-0002-8601-3501
Adam HantmanJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
Gerald M RubinJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.ORCID 0000-0001-8762-8703
Tom KirchhausenJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
Stephan SaalfeldJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.ORCID 0000-0002-4106-1761
Yoshinori AsoJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
Edward S BoydenMIT Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA. esb@media.mit.edu betzige@janelia.hhmi.org.ORCID 0000-0002-0419-3351
Eric BetzigJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA. esb@media.mit.edu betzige@janelia.hhmi.org.ORCID 0000-0002-4192-1274
Howard Hughes Medical Institute · USBoston Children's Hospital · USHarvard University · USIIT@MIT · USMBF Bioscience (United States) · USMcGovern Institute for Brain Research · USUniversity of America · US
Funding
Center for Genomically Engineered OrgansRM1HG008525 · NHGRI · HARVARD MEDICAL SCHOOL · PI CHURCH, GEORGE M · 2015 to 2019
$10.2MNovel Platforms for Systematic Optical Control of Complex Neural Circuits In VivoR01DA029639 · NIDA · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2010 to 2023
$6.7MExpansion MicroscopyR01EB024261 · NIBIB · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2017 to 2025
$4.6MDynamics of Clathrin Coat Formation in CellsR01GM075252 · NIGMS · IMMUNE DISEASE INSTITUTE, INC. · PI KIRCHHAUSEN, TOMAS · 2005 to 2018
$4.6MAutomated 3D quantitative analysis of dendritic spines imaged with light microscopyR44MH093011 · NIMH · MICROBRIGHTFIELD, LLC · PI ANGSTMAN, PAUL · 2012 to 2018
$4.5MMillisecond-Timescale Whole-Brain Neural Activity Mapping in Health and DiseaseDP1NS087724 · NINDS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2013 to 2017
$3.9MHigh-Performance Imaging Through Scattering Living TissueR01DA045549 · NIDA · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2017 to 2020
$3.5MAn Accessible Optical Toolbox for Saturated Nanoscale Analysis of Neural ArchitectureR01MH110932 · NIMH · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI BOYDEN, EDWARD S., CAI, DAWEN · 2016 to 2019
$2.8MScalable Cell- and Circuit-Targeted ElectrophysiologyR01NS102727 · NINDS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S., FOREST, CRAIG · 2017 to 2020
$2.2MThe mechanism of inner ear pressure homeostasis by the endolymphatic sacR01DC015478 · NIDCD · HARVARD MEDICAL SCHOOL · PI MEGASON, SEAN G · 2017 to 2021
$2.0MCausal Analysis of Electrically Connected Neural NetworksR01NS087950 · NINDS · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI HAN, XUE · 2013 to 2016
$1.3MWhole Brain Nanoscale Preservation/ImagingR41MH112318 · NIMH · NECTOME, INC. · PI MCINTYRE, ROBERT · 2016 to 2017
$916kNHGRI NIH HHS RM1 HG008525NIBIB NIH HHS R01 EB024261NIDA NIH HHS R01 DA029639NIDA NIH HHS R01 DA045549NIDCD NIH HHS R01 DC015478NIGMS NIH HHS R01 GM075252NIGMS NIH HHS R25 GM103792NIMH NIH HHS R01 MH110932NIMH NIH HHS R41 MH112318NIMH NIH HHS R44 MH093011NINDS NIH HHS DP1 NS087724NINDS NIH HHS R01 NS087950NINDS NIH HHS R01 NS102727
Abstract
Optical and electron microscopy have made tremendous inroads toward understanding the complexity of the brain. However, optical microscopy offers insufficient resolution to reveal subcellular details, and electron microscopy lacks the throughput and molecular contrast to visualize specific molecular constituents over millimeter-scale or larger dimensions. We combined expansion microscopy and lattice light-sheet microscopy to image the nanoscale spatial relationships between proteins across the thickness of the mouse cortex or the entire
Indexed as
NanotechnologyAnimalsAxonsBrainDendritic SpinesDrosophilaFemaleHumansImage Processing, Computer-AssistedImaging, Three-DimensionalKidneyMaleMiceMice, Inbred C57BLMice, TransgenicMicroscopy, Fluorescence
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