ArticleBMC veterinary research2019
Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2.
Article in BMC veterinary research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- A comprehensive review of lateral flow assays in rapid livestock and poultry disease diagnostics.Journal of advanced veterinary and animal research · 2026Article
- Establishment and application of a fluorescence-based nucleic acid detection system for canine parvovirus.Frontiers in veterinary science · 2025Article
- Genetic Diversity and Recombination Analysis of Canine Parvoviruses Prevalent in Central and Eastern China, from 2020 to 2023.Microorganisms · 2024Article
- The surface modification of the silica-coated magnetic nanoparticles and their application in molecular diagnostics of virus infection.Scientific reports · 2024Article
- Accelerated Development of a COVID-19 Lateral Flow Test in an Academic Setting: Lessons Learned.Accounts of chemical research · 2024Article
- Rapid quantitative detection ofFrontiers in bioengineering and biotechnology · 2023Article
- Glowstick-inspired smartphone-readable reporters for sensitive, multiplexed lateral flow immunoassays.Communications engineering · 2023Article
- Nanoparticle-Based Visual Detection of Amplified DNA for Diagnosis of Hepatitis C Virus.Biosensors · 2022Article
- Facile, Rapid, and Low-Cost Detection for Influenza Viruses and Respiratory Syncytial Virus Based on a Catalytic DNA Assembly Circuit.ACS omega · 2022Article
- Article
- Development of a PCR Lateral Flow Assay for Rapid Detection of Bacillus anthracis, the Causative Agent of Anthrax.Molecular biotechnology · 2021Article
- Genetic Characterisation and Local Genotypes of Canine Parvovirus Strains Collected from Pet Dogs in Central and Eastern China During 2018-2019.Journal of veterinary research · 2020Article
- Development of Solid-Phase RPA on a Lateral Flow Device for the Detection of Pathogens Related to Sepsis.Sensors (Basel, Switzerland) · 2020Article
- Application of magnetic nanoparticles in nucleic acid detection.Journal of nanobiotechnology · 2020Review
- Advances in the Diagnosis of Foot-and-Mouth Disease.Frontiers in veterinary science · 2020Review
- Recent Trends in Nanomaterial-Based Biosensors for Point-of-Care Testing.Frontiers in chemistry · 2020Article
- An Improved Polymerase Cross-Linking Spiral Reaction Assay for Rapid Diagnostic of Canine Parvovirus 2 Infection.Frontiers in veterinary science · 2020Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
backgroundCanine parvovirus 2 (CPV-2) is one of the most common etiological agents that cause severe gastroenteritis in puppies. Early accurate diagnosis is important for infected dogs. In recent years, magnetic separation has become an efficient and useful tool for bioassays. In this study, polymerase chain reaction (PCR) combined with fluorescent lateral flow immunoassay (LFIA) based on magnetic purification assay was developed for the quantitative detection of CPV-2.
resultsThe optimum working reaction volume and reaction time for LFIA was 100 μL and 2 min, respectively. The PCR-LFIA assay only detected CPV-2, and did not show cross-detection of non-CPV strains. Experiments showed analytical sensitivity of 3 × 10
conclusionsThis approach provides an attractive platform for rapid and quantitative detection of CPV-2, indicating great promise as a convenient molecular detection tool to facilitate disease outbreak investigations and response timely.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.