ArticleMicrobial cell factories2019
Combinatorial approach for improved cyanidin 3-O-glucoside production in Escherichia coli.
Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 57 citations in OpenAlex.
- Unlocking cyanidin-3-glucoside potentials with green technologies: advances in extraction, bioavailability, and stability for therapeutic and non-therapeutic applications.Frontiers in nutrition · 2026Review
- Structures, Biological Activities, and Food Industry Applications of Anthocyanins Sourced from Three Berry Plants from the Qinghai-Tibet Plateau.Foods (Basel, Switzerland) · 2025Review
- Comprehensive engineering of novel glycosyltransferase for efficient, donor-promiscuous, and regioselective glycosylation of flavonoids.Science advances · 2025Article
- Advances in Flavonoid Research: Sources, Biological Activities, and Developmental Prospectives.Current issues in molecular biology · 2024Review
- A major facilitator superfamily transporter MdtH inApplied and environmental microbiology · 2024Article
- Biosynthesis of melatonin from L-tryptophan by an engineered microbial cell factory.Biotechnology for biofuels and bioproducts · 2024Article
- Green Solvents for Extraction of Natural Food Colorants from Plants: Selectivity and Stability Issues.Foods (Basel, Switzerland) · 2024Review
- Utilization of carbon catabolite repression for efficiently biotransformation of anthraquinone O-glucuronides byFrontiers in microbiology · 2024Article
- Identification ofFrontiers in microbiology · 2024Article
- Metabolic engineering for the biosynthesis of bis-indolylquinone terrequinone A in Escherichia coli from L-tryptophan and prenol.Biotechnology for biofuels and bioproducts · 2023Article
- Flavonoid Production: Current Trends in Plant Metabolic Engineering and De Novo Microbial Production.Metabolites · 2023Review
- Insight into the Progress on Natural Dyes: Sources, Structural Features, Health Effects, Challenges, and Potential.Molecules (Basel, Switzerland) · 2022Review
- Metabolic engineering of Escherichia coli for efficient degradation of 4-fluorophenol.AMB Express · 2022Article
- Anthocyanins: Traditional Uses, Structural and Functional Variations, Approaches to Increase Yields and Products' Quality, Hepatoprotection, Liver Longevity, and Commercial Products.International journal of molecular sciences · 2022Review
- Biotechnological advances for improving natural pigment production: a state-of-the-art review.Bioresources and bioprocessing · 2022Review
- Efficient production of anthocyanins inFrontiers in bioengineering and biotechnology · 2022Article
- High-level de novo biosynthesis of glycosylated zeaxanthin and astaxanthin in Escherichia coli.Bioresources and bioprocessing · 2021Article
- Metabolic Engineering of Microbial Cell Factories for Biosynthesis of Flavonoids: A Review.Molecules (Basel, Switzerland) · 2021Review
- Metabolic Engineering of Microorganisms for the Production of Flavonoids.Frontiers in bioengineering and biotechnology · 2020Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMulti-monocistronic and multi-variate vectors were designed, built, and tested for the improved production of cyanidin 3-O-glucoside (C3G) in Escherichia coli BL21 (DE3). The synthetic bio-parts were designed in such a way that multiple genes can be assembled using the bio-brick system, and expressed under different promoters in a single vector. The vectors harbor compatible cloning sites, so that the genes can be shuffled from one vector to another in a single step, and assembled into a single vector. The two required genes: anthocyanidin synthase (PhANS) from Petunia hybrida, and cyanidin 3-O-glucosyltransferase (At3GT) from Arabidopsis thaliana, were individually cloned under P
resultsThe engineered strains endowed with newly generated vectors and the genes for C3G biosynthesis and UDP-D-glucose synthesis were fed with 2 mM (+)-catechin and D-glucose for the production of cyanidin, and its subsequent conversion to C3G. One of the engineered strains harboring At3GT and PhANS under P
conclusionA synthetic vector system using different promoters has been developed and used for the synthesis of C3G in E. coli BL21 (DE3) by directing the metabolic flux towards the UDP-D-glucose. This system has the potential of generating better strains for the synthesis of valuable natural products.
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