ArticleCell cycle (Georgetown, Tex.)2019
NEAT1 regulates Th2 cell development by targeting STAT6 for degradation.
Article in Cell cycle (Georgetown, Tex.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Post-translational modifications and immune responses in liver cancer.Frontiers in immunology · 2023Review
- Association of long non-coding RNAs NEAT1, and MALAT1 expression and pathogenesis of Behçet's disease among Egyptian patients.Saudi journal of biological sciences · 2022Article
- Aberrant expressions of circulating lncRNA NEAT1 and microRNA-125a are linked with Th2 cells and symptom severity in pediatric allergic rhinitis.Journal of clinical laboratory analysis · 2022Article
- STAT6 Upregulates NRP1 Expression in Endothelial Cells and Promotes Angiogenesis.Frontiers in oncology · 2022Article
- RNA Sequencing of CD4Journal of molecular neuroscience : MN · 2021Article
- Single-cell characterization of a model of poly I:C-stimulated peripheral blood mononuclear cells in severe asthma.Respiratory research · 2021Article
- Potential impact and mechanism of Long Non-coding RNAs on cancer and associated T cells.Journal of Cancer · 2021Review
- Long non-coding RNA NEAT1-centric gene regulation.Cellular and molecular life sciences : CMLS · 2020Review
- The Long Non-coding RNAs: Paramount Regulators of the NLRP3 Inflammasome.Frontiers in immunology · 2020Review
- LncRNAs as Chromatin Regulators in Cancer: From Molecular Function to Clinical Potential.Cancers · 2019Review
- Long Non-Coding RNA Function in CD4Non-coding RNA · 2019Review
- Activation of the IL-4/STAT6 Signaling Pathway Promotes Lung Cancer Progression by Increasing M2 Myeloid Cells.Frontiers in immunology · 2019Article
Corrections and comments
- Retraction · 2020-09-10Error in Data ·
- Retracted
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to investigate the role of lncRNA NEAT1 (nuclear enriched abundant transcript 1) in regulating Th2 cell differentiation. The overexpression vectors of NEAT1 and ITCH, and siRNA targeting NEAT1, EZH2 (enhancer of zeste homolog 2) and STAT6 (signal transducer and activator of transcription 6) were transfected into CD4+T cells. The mRNA expressions of ITCH and STAT6 were analyzed by qRT-PCR and western blotting. The levels of Th2 cytokines were detected by ELISA assay. RIP and ChIP assays were performed to analyze the association between NEAT1 and EZH2 as well as EZH2 and STAT6, respectively. Results showed that NEAT1 significantly repressed ITCH expression and increased STAT6 expression as well as the levels of IL-4, IL-5 and IL-13 in CD4+T cells. RIP and ChIP assays revealed that NEAT1 bound to EZH2 and EZH2 was recruited to the promoter region of ITCH in CD4+T cells. Silencing EZH2 significantly promoted STAT6 for ubiquitination. Furthermore, NEAT targeted STAT6 for ubiquitination and elevated levels of Th2 cytokines by regulating EZH2/ITCH axis. In conclusion, our data indicated that NEAT1 promotes Th2 cell differentiation through the EZH2/ITCH/STAT6 axis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.