ArticleViruses2019
A Rapid Method for Sequencing Double-Stranded RNAs Purified from Yeasts and the Identification of a Potent K1 Killer Toxin Isolated from
Article in Viruses, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Alanine scanning of the yeast killer toxin K2 reveals key residues for activity, gain-of-function variants, and supports prediction of precursor processing and 3D structure.Journal of structural biology: X · 2026Article
- Extrachromosomally co-encoded toxin-immunity pairs in yeast and their application potential.Applied microbiology and biotechnology · 2026Review
- Article
- Novel killer yeasts and toxins from the gardens of fungus-growing ants.Applied and environmental microbiology · 2026Article
- ThemBio · 2025Article
- Totivirus-satellite coinfection prevalence and host genotype associations in wild Saccharomyces cerevisiae.FEMS microbiology ecology · 2025Article
- A Comprehensive Structural and Functional Analysis ofbioRxiv : the preprint server for biology · 2025Article
- An empirical long-term competition among natural yeast isolates reveals that short-term fitness largely but not entirely predicts long-term outcomes.bioRxiv : the preprint server for biology · 2025Article
- Aspergillus fumigatus dsRNA virus promotes fungal fitness and pathogenicity in the mammalian host.Nature microbiology · 2025Article
- An optimized protocol for quality control of gene therapy vectors using nanopore direct RNA sequencing.Genome research · 2024Article
- Inhibition of diastatic yeasts byApplied and environmental microbiology · 2024Article
- Uncovering the Yeast Communities in Fungus-Growing Ant Colonies.Microbial ecology · 2023Article
- Tracking Mycoviruses in Public RNAseq Datasets ofViruses · 2023Article
- Novel viruses of the family Partitiviridae discovered in Saccharomyces cerevisiae.PLoS pathogens · 2023Article
- The prevalence of killer yeasts and double-stranded RNAs in the budding yeast Saccharomyces cerevisiae.FEMS yeast research · 2023Article
- Expression of the K74 Killer Toxin from Saccharomyces paradoxus Is Modulated by the Toxin-Encoding M74 Double-Stranded RNA 5' Untranslated Terminal Region.Applied and environmental microbiology · 2022Article
- A Simple Multiplex Reverse Transcription-PCR Method for the Diagnosis of L-A and M Totiviruses in Saccharomyces cerevisiae.Applied and environmental microbiology · 2022Article
- Article
- A Sustainable Alternative for Postharvest Disease Management and Phytopathogens Biocontrol in Fruit: Antagonistic Yeasts.Plants (Basel, Switzerland) · 2021Review
- Vaginal Isolates of Candida glabrata Are Uniquely Susceptible to Ionophoric Killer Toxins Produced by Saccharomyces cerevisiae.Antimicrobial agents and chemotherapy · 2021Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Mycoviruses infect a large number of diverse fungal species, but considering their prevalence, relatively few high-quality genome sequences have been determined. Many mycoviruses have linear double-stranded RNA genomes, which makes it technically challenging to ascertain their nucleotide sequence using conventional sequencing methods. Different specialist methodologies have been developed for the extraction of double-stranded RNAs from fungi and the subsequent synthesis of cDNAs for cloning and sequencing. However, these methods are often labor-intensive, time-consuming, and can require several days to produce cDNAs from double-stranded RNAs. Here, we describe a comprehensive method for the rapid extraction and sequencing of dsRNAs derived from yeasts, using short-read next generation sequencing. This method optimizes the extraction of high-quality double-stranded RNAs from yeasts and 3' polyadenylation for the initiation of cDNA synthesis for next-generation sequencing. We have used this method to determine the sequence of two mycoviruses and a double-stranded RNA satellite present within a single strain of the model yeast
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