ReviewCells2019
Cellular and Structural Studies of Eukaryotic Cells by Cryo-Electron Tomography.
Review in Cells, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Macromolecular Crowding in Cytoplasm and Cellular Organelles.Sub-cellular biochemistry · 2025Pooled it
- High-resolution cryoEM of nucleosomes in nuclear extracts of mammalian cells.Bioscience reports · 2026Article
- The Actin Network Interfacing Diverse Integrin-Mediated Adhesions.Biomolecules · 2023Review
- CryomicroscopyFaraday discussions · 2022Article
- Cryo-electron tomography with mixed-scale dense neural networks reveals key steps in deployment ofPNAS nexus · 2022Article
- Article
- Using Cryo-ET to distinguish platelets during pre-acute myeloid leukemia from steady state hematopoiesis.Communications biology · 2022Article
- A cryo-TSEM with temperature cycling capability allows deep sublimation of ice to uncover fine structures in thick cells.Scientific reports · 2021Article
- Structural analysis of receptors and actin polarity in platelet protrusions.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Structural heterogeneity of cellular K5/K14 filaments as revealed by cryo-electron microscopy.eLife · 2021Article
- Unveiling the polarity of actin filaments by cryo-electron tomography.Structure (London, England : 1993) · 2021Article
- A lamin A/C variant causing striated muscle disease provides insights into filament organization.Journal of cell science · 2021Article
- DNA origami signposts for identifying proteins on cell membranes by electron cryotomography.Cell · 2021Article
- Article
- Development of Styrene Maleic Acid Lipid Particles as a Tool for Studies of Phage-Host Interactions.Journal of virology · 2020Article
- Segmentation by classification: A novel and reliable approach for semi-automatic selection of HIV/SIV envelope spikes.Journal of structural biology · 2020Article
- Considerations of Antibody Geometric Constraints on NK Cell Antibody Dependent Cellular Cytotoxicity.Frontiers in immunology · 2020Review
- Novel Secondary Ion Mass Spectrometry Methods for the Examination of Metabolic Effects at the Cellular and Subcellular Levels.Frontiers in behavioral neuroscience · 2020Article
- Ultrastructural cellular signatures: does cellular form follow function?National science review · 2019Article
- A collection of yeast cellular electron cryotomography data.GigaScience · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The architecture of protein assemblies and their remodeling during physiological processes is fundamental to cells. Therefore, providing high-resolution snapshots of macromolecular complexes in their native environment is of major importance for understanding the molecular biology of the cell. Cellular structural biology by means of cryo-electron tomography (cryo-ET) offers unique insights into cellular processes at an unprecedented resolution. Recent technological advances have enabled the detection of single impinging electrons and improved the contrast of electron microscopic imaging, thereby significantly increasing the sensitivity and resolution. Moreover, various sample preparation approaches have paved the way to observe every part of a eukaryotic cell, and even multicellular specimens, under the electron beam. Imaging of macromolecular machineries at high resolution directly within their native environment is thereby becoming reality. In this review, we discuss several sample preparation and labeling techniques that allow the visualization and identification of macromolecular assemblies in situ, and demonstrate how these methods have been used to study eukaryotic cellular landscapes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.