ArticleExperimental eye research2019
Epithelial cells exert differential traction stress in response to substrate stiffness.
Article in Experimental eye research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Cellular Biological Basis and Novel Breakthroughs in the Pathogenesis of Myopia - A Narrative Review.International journal of general medicine · 2026Review
- Vinculin Y822 phosphorylation regulates adhesion remodeling during cardiomyocyte maturation.Journal of cell science · 2025Article
- LNP-encapsulated miRNA29b for corneal repair: A novel approach to combat fibrosis.Materials today. Bio · 2025Article
- Proximity labeling reveals dynamic changes in the SQSTM1 protein network.bioRxiv : the preprint server for biology · 2024Article
- Influence of Substrate Stiffness on iPSC-Derived Retinal Pigmented Epithelial Cells.Stem cells translational medicine · 2024Article
- Tracking cell layer contribution during repair of the tympanic membrane.Disease models & mechanisms · 2024Article
- The biophysical property of the limbal niche maintains stemness through YAP.Cell death and differentiation · 2023Article
- Contact Guidance Drives Upward Cellular Migration at the Mesoscopic Scale.Cellular and molecular bioengineering · 2023Article
- Corneal optical density: Structural basis, measurements, influencing factors, and roles in refractive surgery.Frontiers in bioengineering and biotechnology · 2023Review
- Age Dependent Changes in Corneal Epithelial Cell Signaling.Frontiers in cell and developmental biology · 2022Article
- Silk films with nanotopography and extracellular proteins enhance corneal epithelial wound healing.Scientific reports · 2021Article
- Review
- Modeling the cornea in 3-dimensions: Current and future perspectives.Experimental eye research · 2020Review
- Polymodal Sensory Transduction in Mouse Corneal Epithelial Cells.Investigative ophthalmology & visual science · 2020Article
- Changes in Epithelial and Stromal Corneal Stiffness Occur with Age and Obesity.Bioengineering (Basel, Switzerland) · 2020Article
- Modeling of Mechanosensing Mechanisms Reveals Distinct Cell Migration Modes to Emerge From Combinations of Substrate Stiffness and Adhesion Receptor-Ligand Affinity.Frontiers in bioengineering and biotechnology · 2020Article
- Influence of polydimethylsiloxane substrate stiffness on corneal epithelial cells.Royal Society open science · 2019Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Epithelial wound healing is essential for maintaining the function and clarity of the cornea. Successful repair after injury involves the coordinated movements of cell sheets over the wounded region. While collective migration has been the focus of studies, the effects that environmental changes have on this form of movement are poorly understood. To examine the role of substrate compliancy on multi-layered epithelial sheet migration, we performed traction force and confocal microscopy to determine differences in traction forces and to examine focal adhesions on synthetic and biological substrates. The leading edges of corneal epithelial sheets undergo retraction or contraction prior to migration, and alterations in the sheet's stiffness are affected by the amount of force exerted by cells at the leading edge. On substrates of 30 kPa, cells exhibited greater and more rapid movement than on substrates of 8 kPa, which are similar to that of the corneal basement membrane. Vinculin and its phosphorylated residue Y1065 were prominent along the basal surface of migrating cells, while Y822 was prominent between neighboring cells along the leading edge. Vinculin localization was diffuse on a substrate where the basement membrane was removed. Furthermore, when cells were cultured on fibronectin-coated acrylamide substrates of 8 and 50 kPa and then wounded, there was an injury-induced phosphorylation of Y1065 and substrate dependent changes in the number and size of vinculin containing focal adhesions. These results demonstrate that changes in substrate stiffness affected traction forces and vinculin dynamics, which potentially could contribute to the delayed healing response associated with certain corneal pathologies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.