ReviewMicrobial cell (Graz, Austria)2019
Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways.
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In one paragraph
Review in Microbial cell (Graz, Austria), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35citing papers in PubMed
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Who cites it
35 citing papers in PubMed.
- DNA-damage dependent interaction of Orc6 to SMARCA1 in S-phase modulates chromatin remodeling.Nucleic acids research · 2026Article
- Potential Rad54 separation of function mutation highlights unique roles during homologous recombination.PLoS genetics · 2026Article
- RecBCD-dependent post-UV replication restart inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Mitotic Heteroallelic and Sister Chromatid Recombination.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Comparative genomics uncovers adaptive and biotechnological potential of Enterobacter xiangfangensis MDMC82 isolated from desert, and highlights putative core Enterobacteriaceae functions.Scientific reports · 2025Article
- Formation of chromosomal rearrangements inbioRxiv : the preprint server for biology · 2025Article
- Concise Overview of Methodologies Employed in the Study of Bacterial DNA Replication.International journal of molecular sciences · 2025Review
- Yeast Tools for Studying Type II Topoisomerases in Budding Yeast.Methods in molecular biology (Clifton, N.J.) · 2025Article
- The translocation activity of Rad54 reduces crossover outcomes during homologous recombination.Nucleic acids research · 2024Article
- CRISPR/Cas9 as a Mutagenic Factor.International journal of molecular sciences · 2024Review
- Back-Up Base Excision DNA Repair in Human Cells Deficient in the Major AP Endonuclease, APE1.International journal of molecular sciences · 2023Article
- Rad53 regulates the lifetime of Rdh54 at homologous recombination intermediates.Nucleic acids research · 2023Article
- Nonlethal Furfural Exposure Causes Genomic Alterations and Adaptability Evolution in Saccharomyces cerevisiae.Microbiology spectrum · 2023Article
- Deacetylation induced nuclear condensation of HP1γ promotes multiple myeloma drug resistance.Nature communications · 2023Article
- Article
- Transgenic mice harboring direct repeat substrates reveal key underlying causes of homologous recombination in vivo.DNA repair · 2022Review
- Rdh54 stabilizes Rad51 at displacement loop intermediates to regulate genetic exchange between chromosomes.PLoS genetics · 2022Article
- Thymine-starvation-induced chromosomal fragmentation is not required for thymineless death in Escherichia coli.Molecular microbiology · 2022Article
- Uncovering Bleomycin-Induced Genomic Alterations and Underlying Mechanisms in the YeastApplied and environmental microbiology · 2022Article
- Technical Evaluation of Commercial Sperm DFI Quality Control Products in SCSA Testing.Journal of healthcare engineering · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
48 authors.
Hannah L KleinDepartment of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY, USA.
Giedrė BačinskajaDepartment of Biology, University of Copenhagen, DK-2200 Copenhagen N, Denmark.
Jun CheDepartment of Radiation Oncology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, USA.
Anais CheblalFriedrich Miescher Institute for Biomedical Research (FMI), 4058 Basel, Switzerland.
Rajula ElangoDepartment of Biology, University of Iowa, Iowa City, IA, USA.
Anastasiya EpshteinDepartment of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY, USA.
Devon M FitzgeraldDan L Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, TX, USA.
Belén Gómez-GonzálezCentro Andaluz de BIología Molecular y Medicina Regenerativa-CABIMER, Universidad de Sevilla, Seville, Spain.
Sharik R KhanDepartment of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Sandeep KumarDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
Bryan A LelandYale School of Medicine, New Haven, CT, USA.
Léa MarieDepartment of Microbiology and Immunology, Columbia University Medical Center, New York, NY, USA.
Qian MeiSystems, Synthetic and Physical Biology Graduate Program, Rice University, Houston, TX, USA.
Judith Miné-HattabInstitut Curie, PSL Research University, CNRS, UMR3664, F-75005 Paris, France.
Alicja PiotrowskaNanoBioMedical Centre, Faculty of Physics, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland.
Erica J PolleysDepartment of Biology, Tufts University, Medford, MA USA.
Christopher D PutnamLudwig Institute for Cancer Research, University of California School of Medicine, San Diego, La Jolla, CA, USA.
Elina A RadchenkoDepartment of Biology, Tufts University, Medford, MA USA.
Anissia Ait SaadaInstitut Curie, PSL Research University, CNRS, UMR3348 F-91405, Orsay, France.
Cynthia J SakofskyGenome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, Durham, NC, USA.
Eun Yong ShimDepartment of Radiation Oncology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, USA.
Mathew StracyDepartment of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.
Jun XiaDan L Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, TX, USA.
Zhenxin YanDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
Yi YinDepartment of Molecular Genetics and Microbiology and University Program in Genetics and Genomics, Duke University Medical Center, Durham, NC USA.
Andrés AguileraCentro Andaluz de BIología Molecular y Medicina Regenerativa-CABIMER, Universidad de Sevilla, Seville, Spain.
Juan Lucas ArguesoDepartment of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO, USA.
Catherine H FreudenreichDepartment of Biology, Tufts University, Medford, MA USA.
Susan M GasserFriedrich Miescher Institute for Biomedical Research (FMI), 4058 Basel, Switzerland.
Dmitry A GordeninGenome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, Durham, NC, USA.
James E HaberDepartment of Biology and Rosenstiel Basic Medical Sciences Research Center Brandeis University, Waltham, MA, USA.
Grzegorz IraDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
Sue Jinks-RobertsonDepartment of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC USA.
Megan C KingYale School of Medicine, New Haven, CT, USA.
Richard D KolodnerLudwig Institute for Cancer Research, University of California School of Medicine, San Diego, La Jolla, CA, USA.
Andrei KuzminovDepartment of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Sarah Ae LambertInstitut Curie, PSL Research University, CNRS, UMR3348 F-91405, Orsay, France.
Sang Eun LeeDepartment of Radiation Oncology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, USA.
Kyle M MillerDan L Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, TX, USA.
Sergei M MirkinDepartment of Biology, Tufts University, Medford, MA USA.
Thomas D PetesDepartment of Molecular Genetics and Microbiology and University Program in Genetics and Genomics, Duke University Medical Center, Durham, NC USA.
Susan M RosenbergDan L Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, TX, USA.
Rodney RothsteinDepartment of Genetics & Development, Columbia University Irving Medical Center, New York, NY, USA.
Lorraine S SymingtonDepartment of Microbiology and Immunology, Columbia University Medical Center, New York, NY, USA.
Pawel ZawadzkiNanoBioMedical Centre, Faculty of Physics, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland.
Nayun KimDepartment of Microbiology and Molecular Genetics, The University of Texas Health Science Center at Houston, Houston, TX, USA.
Michael LisbyDepartment of Biology, University of Copenhagen, DK-2200 Copenhagen N, Denmark.
Anna MalkovaDepartment of Biology, University of Iowa, Iowa City, IA, USA.
Funding
Skill Development for Diverse Scientific Careers: A New Course Predoctoral Program in Cellular and Molecular BiologyT32GM007223 · NIGMS · YALE UNIVERSITY · PI BASERGA, SUSAN J · 1985 to 2019
$31.0MGenome-wide hypermutation and structural instabilityZIAES103266 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI GORDENIN, DMITRY · 2015 to 2025
$18.0MENZYMOLOGY OF MISMATCH REPAIR IN YEASTR01GM050006 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Richard D Kolodner · 1994 to 2026
$10.5MENZYMATIC MECHANISMS OF GENETIC RECOMBINATIONR01GM026017 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Richard D Kolodner · 1985 to 2026
$10.5MDNA Damage Response and Repair of a Broken ChromosomeR35GM127029 · NIGMS · BRANDEIS UNIVERSITY · PI JAMES E HABER · 2018 to 2026
$8.6MMutations Arising During DNA RepairP01GM105473 · NIGMS · BRANDEIS UNIVERSITY · PI LOVETT, SUSAN THOMAS · 2014 to 2018
$8.6MMolecular Mechanisms Underlying Recombination at DNA Double-Strand Breaks and Stalled Replication ForksR35GM118180 · NIGMS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI Rodney J. ROTHSTEIN · 2016 to 2026
$8.5MGenetic regulation of genome stability in yeastR35GM118020 · NIGMS · DUKE UNIVERSITY · PI PETES, THOMAS · 2016 to 2025
$7.0MMechanisms of DNA Motor Proteins in Genome MaintenanceR01CA146940 · NCI · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI KLEIN, HANNAH L. · 2009 to 2019
$5.9MMechanism and regulation of DNA double-strand break repairR35GM126997 · NIGMS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI Lorraine S Symington · 2018 to 2026
$5.9MRegulation of DNA recombination in Saccharomyces cerevisiaeR01GM080600 · NIGMS · BAYLOR COLLEGE OF MEDICINE · PI IRA, GRZEGORZ A · 2007 to 2024
$5.5MREPLICATION OF SIMPLE DNA REPEATSR01GM060987 · NIGMS · UNIVERSITY OF ILLINOIS AT CHICAGO · PI MIRKIN, SERGEI · 2001 to 2017
$4.9MNCI NIH HHS DP1 CA174424NCI NIH HHS R01 CA146940NIEHS NIH HHS R03 ES029306NIGMS NIH HHS P01 GM105473NIGMS NIH HHS R01 GM026017NIGMS NIH HHS R01 GM050006NIGMS NIH HHS R01 GM060987NIGMS NIH HHS R01 GM071011NIGMS NIH HHS R01 GM073115NIGMS NIH HHS R01 GM080600NIGMS NIH HHS R01 GM116007NIGMS NIH HHS R01 GM122880NIGMS NIH HHS R01 GM125650NIGMS NIH HHS R35 GM118020NIGMS NIH HHS R35 GM118077NIGMS NIH HHS R35 GM118180NIGMS NIH HHS R35 GM119788NIGMS NIH HHS R35 GM122598NIGMS NIH HHS R35 GM126997NIGMS NIH HHS R35 GM127006NIGMS NIH HHS R35 GM127029NIGMS NIH HHS R35 GM130322NIGMS NIH HHS T32 GM007223Wellcome Trust 204684/Z/16/Z
Abstract
Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic, molecular, and cytological reporters. The assays are powerful tools but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies.
Indexed as
chromatin dynamicschromosome rearrangementscrossoversDNA breaksDNA repair centersDNA resectionDSBsfluorescent proteinsgene amplificationgene conversiongenome instabilitygross chromosome rearrangementsHolliday junctionshomologous recombinationmitotic recombinationmutagenesispulsed field gel electrophoresisreplication fork stallingR-loopssingle-particle trackingsister chromatid recombinationsister repetitive sequencessite-specific chromosome breakstoxic recombination intermediatesyeast artificial chromosome
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