ArticleExperimental and therapeutic medicine2019
Combined effects of hyperphosphatemia and hyperglycemia on the calcification of cultured human aortic smooth muscle cells.
Article in Experimental and therapeutic medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 29 citations in OpenAlex.
- Advances in the clinical applications of sodium thiosulphate.Annals of medicine · 2026Review
- Review
- Patient characteristics modify the association between changes in mineral metabolism parameters and mortality in a nationwide hemodialysis cohort study.Scientific reports · 2025Article
- Pathophysiology of chronic kidney disease-mineral bone disorder (CKD-MBD): from adaptive to maladaptive mineral homeostasis.Clinical kidney journal · 2025Article
- Review
- Hypoxia-Inducible Factor-1Cardiology research and practice · 2024Article
- Phosphonoformic acid reduces hyperphosphatemia-induced vascular calcification via Pit-1.The Journal of international medical research · 2024Article
- Therapeutic Applications and Effects ofFoods (Basel, Switzerland) · 2023Review
- Lumican silencing ameliorates β-glycerophosphate-mediated vascular smooth muscle cell calcification by attenuating the inhibition of APOB on KIF2C activity.Open medicine (Warsaw, Poland) · 2023Article
- Both high glucose and phosphate overload promote senescence-associated calcification of vascular muscle cells.International urology and nephrology · 2022Article
- Plasma phosphate and all-cause mortality in individuals with and without type 2 diabetes: the Dutch population-based lifelines cohort study.Cardiovascular diabetology · 2022Article
- Cardiovascular benefits from SGLT2 inhibition in type 2 diabetes mellitus patients is not impaired with phosphate flux related to pharmacotherapy.World journal of cardiology · 2021Review
- Association between metabolic parameters and risks of anemia and electrolyte disturbances among stages 3-5 chronic kidney disease patients in Taiwan.BMC nephrology · 2021Article
- BGP-15 Inhibits Hyperglycemia-Aggravated VSMC Calcification Induced by High Phosphate.International journal of molecular sciences · 2021Article
- Iron Sucrose: A Double-Edged Sword in High Phosphate Media-Induced Vascular Calcification.Calcified tissue international · 2021Article
- Dietary Phosphorus as a Marker of Mineral Metabolism and Progression of Diabetic Kidney Disease.Nutrients · 2021Review
- RAGE Differentially AlteredFrontiers in physiology · 2021Article
- Role of SGK1 in the Osteogenic Transdifferentiation and Calcification of Vascular Smooth Muscle Cells Promoted by Hyperglycemic Conditions.International journal of molecular sciences · 2020Article
- The NovelInternational journal of molecular sciences · 2020Article
- Selenium-Containing Protein From Selenium-EnrichedFrontiers in physiology · 2020Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vascular calcification (VC) is common in patients with diabetes and/or chronic kidney disease (CKD). It is strongly associated with cardiovascular morbidity and mortality. Hyperphosphatemia caused by CKD induces the transformation of vascular smooth muscle cells (VSMCs) into chondrocytes or osteoblast-like cells. Hyperglycemia may also accelerate VC. However, the exact mechanisms of this remain unclear. The effects of simultaneous hyperphosphatemia and hyperglycemia require investigation. CKD rat models are typically used to study VC, which are far removed from the clinical situations of patients with CKD. The present study cultured human aortic smooth muscle cells (HASMCs) in normal, hyperphosphatemic and/or hyperglycemic conditions for 14 days. Alizarin red staining, calcification content, VSMC differentiation marker gene expression, phenotypic osteoblast gene expression and type III sodium-dependent phosphate cotransporter-1 (Pit-1) protein expression was examined. Hyperphosphatemia and hyperglycemia had combined effects in promoting calcification, phenotypic transition and Pit-1 expression in cultured HASMCs. In the present study, the combined effects of hyperphosphatemia and hyperglycemia on the calcification and phenotypic transition of HASMCs were demonstrated. Hyperphosphatemia combined with hyperglycemia medium should be considered an appropriate experimental model to study VC in diabetic kidney disease (DKD). Pit-1 should be considered as a promising index of VC.
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