Evidence map›Paper›PMID 30651536›Full record

ArticleNature communications2019

Regulation of in vivo dynein force production by CDK5 and 14-3-3ε and KIAA0528.

Dail E Chapman, Babu J N Reddy, Bunchhin Huy, Matthew J Bovyn, Stephen John S Cruz, Zahraa M Al-Shammari, Han Han, Wenqi Wang, Deanna S Smith, Steven P Gross

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.7field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 24 citations in OpenAlex.

  1. Article
  2. Article
  3. MARK2 phosphorylates KIF13A at a 14-3-3 binding site to polarize vesicular transport of transferrin receptor within dendrites.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Lipid droplet motility and organelle contacts.Contact (Thousand Oaks (Ventura County, Calif.))
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Dail E ChapmanDevelopmental and Cell Biology and Physics, University of California, Irvine, CA, USA.
Babu J N ReddyDevelopmental and Cell Biology and Physics, University of California, Irvine, CA, USA.
Bunchhin HuyDevelopmental and Cell Biology and Physics, University of California, Irvine, CA, USA.
Matthew J BovynDevelopmental and Cell Biology and Physics, University of California, Irvine, CA, USA.ORCID http://orcid.org/0000-0001-9331-4583
Stephen John S CruzDevelopmental and Cell Biology and Physics, University of California, Irvine, CA, USA.
Zahraa M Al-ShammariDevelopmental and Cell Biology and Physics, University of California, Irvine, CA, USA.
Han HanDevelopmental and Cell Biology and Physics, University of California, Irvine, CA, USA.ORCID http://orcid.org/0000-0003-2222-5270
Wenqi WangDevelopmental and Cell Biology and Physics, University of California, Irvine, CA, USA.ORCID http://orcid.org/0000-0003-4053-5088
Deanna S SmithBiological Sciences, University of South Carolina, Columbia, SC, USA.ORCID http://orcid.org/0000-0002-3014-641X
Steven P GrossDevelopmental and Cell Biology and Physics, University of California, Irvine, CA, USA. sgross@uci.edu.
University of California, Irvine · USUniversity of South Carolina · US

Funding

Control of cargo distributions by microtubule motor physical interactions with cargo, cytoplasm and MAPsR01GM123068 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI ALLARD, JUN · 2017 to 2021
$1.7M
NIGMS NIH HHS R01 GM123068NIH HHS R01 GM123068
6 · The paper itself

Abstract

Single-molecule cytoplasmic dynein function is well understood, but there are major gaps in mechanistic understanding of cellular dynein regulation. We reported a mode of dynein regulation, force adaptation, where lipid droplets adapt to opposition to motion by increasing the duration and magnitude of force production, and found LIS1 and NudEL to be essential. Adaptation reflects increasing NudEL-LIS1 utilization; here, we hypothesize that such increasing utilization reflects CDK5-mediated NudEL phosphorylation, which increases the dynein-NudEL interaction, and makes force adaptation possible. We report that CDK5, 14-3-3ε, and CDK5 cofactor KIAA0528 together promote NudEL phosphorylation and are essential for force adaptation. By studying the process in COS-1 cells lacking Tau, we avoid confounding neuronal effects of CDK5 on microtubules. Finally, we extend this in vivo regulatory pathway to lysosomes and mitochondria. Ultimately, we show that dynein force adaptation can control the severity of lysosomal tug-of-wars among other intracellular transport functions involving high force.

Indexed as

14-3-3 Proteins1-Alkyl-2-acetylglycerophosphocholine EsteraseAnimalsBiomechanical PhenomenaCarrier ProteinsChlorocebus aethiopsCOS CellsCyclin-Dependent Kinase 5Cytoplasmic DyneinsLipid DropletsLysosomesMicrotubule-Associated ProteinsMicrotubulesMitochondriaRNA, Small Interfering14-3-3 Proteins1-Alkyl-2-acetylglycerophosphocholine EsteraseC2CD5 protein, humanCarrier ProteinsCDK5 protein, humanCyclin-Dependent Kinase 5Cytoplasmic DyneinsMicrotubule-Associated ProteinsNDEL1 protein, humanPAFAH1B1 protein, humanRNA, Small InterferingYWHAE protein, human

Identifiers

PMID30651536
PMCPMC6335402
OpenAlexW2910780591

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.