ArticleThe Journal of biological chemistry2019
The kinetic mechanisms of fast-decay red-fluorescent genetically encoded calcium indicators.
Article in The Journal of biological chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Article
- Fluorescent sensors for intracellular signaling in the brain: imaging neurons, glia, and vascular cells.Neurophotonics · 2025Article
- Mechanical Modulation, Physiological Roles, and Imaging Innovations of Intercellular Calcium Waves in Living Systems.Cancers · 2025Review
- Far-red fluorescent genetically encoded calcium ion indicators.Nature communications · 2025Article
- Photopharmacology reveals high-specificity linkage of CaFrontiers in immunology · 2025Article
- BDNF-dependent nano-organization of Neogenin and the WAVE regulatory complex promotes actin remodeling in dendritic spines.iScience · 2024Article
- Seizure event detection using intravital two-photon calcium imaging data.Neurophotonics · 2024Article
- Extracellular glutamate and GABA transients at the transition from interictal spiking to seizures.Brain : a journal of neurology · 2024Article
- Subcellular Imaging of Neuronal Calcium Handling In Vivo.Journal of visualized experiments : JoVE · 2023Article
- Development and characterization of novel jGCaMP8f calcium sensor variants with improved kinetics and fluorescence response range.Frontiers in cellular neuroscience · 2023Article
- Genetically encoded fluorescent biosensors for GPCR research.Frontiers in cell and developmental biology · 2022Review
- The Next Frontier for Designing Switchable Proteins: Rational Enhancement of Kinetics.The journal of physical chemistry. B · 2021Article
- Optimizing Calcium Detection Methods in Animal Systems: A Sandbox for Synthetic Biology.Biomolecules · 2021Review
- Using Genetically Encoded Calcium Indicators to Study Astrocyte Physiology: A Field Guide.Frontiers in cellular neuroscience · 2021Article
- Design of Calcium-Binding Proteins to Sense Calcium.Molecules (Basel, Switzerland) · 2020Review
- Optically activated, customizable, excitable cells.PloS one · 2020Article
- Red Photoactivatable Genetic Optical-Indicators.Frontiers in cellular neuroscience · 2020Article
- Single-Cell Optogenetic Control of Calcium Signaling with a High-Density Micro-LED Array.iScience · 2019Article
- Understanding the Fluorescence Change in Red Genetically Encoded Calcium Ion Indicators.Biophysical journal · 2019Article
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5 authors.
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Abstract
Genetically encoded calcium indicators (GECIs) are useful reporters of cell-signaling, neuronal, and network activities. We have generated novel fast variants and investigated the kinetic mechanisms of two recently developed red-fluorescent GECIs (RGECIs), mApple-based jRGECO1a and mRuby-based jRCaMP1a. In the formation of fluorescent jRGECO1a and jRCaMP1a complexes, calcium binding is followed by rate-limiting isomerization. However, fluorescence decay of calcium-bound jRGECO1a follows a different pathway from its formation: dissociation of calcium occurs first, followed by the peptide, similarly to GCaMP-s. In contrast, fluorescence decay of calcium-bound jRCaMP1a occurs by the reversal of the on-pathway: peptide dissociation is followed by calcium. The mechanistic differences explain the generally slower off-kinetics of jRCaMP1a-type indicators compared with GCaMP-s and jRGECO1a-type GECI: the fluorescence decay rate of f-RCaMP1 was 21 s
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