Evidence map›Paper›PMID 30647689›Full record

ArticleJournal, genetic engineering & biotechnology2017

First study of sperm mediated gene transfer in Egyptian river buffalo.

Mohamed S Hassanane, Aida I El Makawy, Sahar M Helalia, Ahmed S Abdoon, Kamal M A Khalil, Tharwat A Ghanem, Amany M Tohamy, Xiao-Feng Sun, Wei Shen

Abstract read
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In one paragraph

Article in Journal, genetic engineering & biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Mohamed S HassananeCell Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre, Egypt.
Aida I El MakawyCell Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre, Egypt.
Sahar M HelaliaCell Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre, Egypt.
Ahmed S AbdoonAnimal Reproduction and Artificial Insemination Department, Veterinary Research Division, National Research Centre, Egypt.
Kamal M A KhalilGenetics and Cytology Dept., Genetic Engineering and Biotechnology Division, National Research Centre, Egypt.
Tharwat A GhanemZoology Department, Faculty of Science, Helwan University, Egypt.
Amany M TohamyZoology Department, Faculty of Science, Helwan University, Egypt.
Xiao-Feng SunInstitute of Reproductive Sciences, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.
Wei ShenInstitute of Reproductive Sciences, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present study was carried out to find the best treatments for enhancing the ration of insertion of a desired gene construct (pEGFP-N1) onto the sperm of buffalo as the first step for the production of transgenic buffalo using sperm mediated gene transfer (SMGT). The tested conditions were plasmid DNA concentration, sperm concentration, transfecting agent concentration: Dimethyle sulphoxide (DMSO) and time of transfection. The study proved that the best conditions for producing transgenic embryos were incubation sperm solution its concentration is 10

Indexed as

ANOVA, analysis of varianceBuffalo embryosCCC, covalently closed circularCOCs, Cumulus oocyte complexesDMSODMSO, Dimethyle sulphoxideEGFPEGFP, enhanced green fluorescent proteinIVF, in vitro fertilizationMII, second meiotic divisionOD, optical densityPBS, Phosphate buffer salineSMGTSMGT, sperm mediated gene transferTCM199, tissue culture mediumTransgenic

Identifiers

PMID30647689
PMCPMC6296624

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.