Evidence map›Paper›PMID 30645735›Full record

ArticleActa parasitologica2019

Molecular Characterization of Chitin Synthase Gene of Demodex canis from Mizoram, India.

Gautam Patra, Parthasarathi Behera, Subhamoy Ghosh, Debashish Mohanta, Sonjoy Kumar Borthakur, Papia Biswas, Ajit Kumar, Apurba Debbarma

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Article in Acta parasitologica, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Open veterinary journal · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Gautam PatraDepartment of Veterinary Parasitology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, India. dr.gautampatra@yahoo.co.in.
Parthasarathi BeheraDepartment of Veterinary Physiology and Biochemistry, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, India.
Subhamoy GhoshDepartment of Veterinary Parasitology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, India.
Debashish MohantaState Animal Health Centre, Bhatar, Purba Bardhaman, West Bengal, India.
Sonjoy Kumar BorthakurDepartment of Veterinary Parasitology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, India.
Papia BiswasDepartment of Veterinary Public Health and Epidemiology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, India.
Ajit KumarDepartment of Veterinary Parasitology, F/O-VAS, WBUAFS, Kolkata, West Bengal, India.
Apurba DebbarmaDepartment of Veterinary Parasitology, College of Veterinary Sciences and Animal Husbandry, R.K. Nagar, Agartala, Tripura, India.
Central Agricultural University · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimsCanine demodicosis is a parasitic condition affecting the skin of dogs. The present study was designed to characterize chitin synthase gene of Demodex canis. The molecular technique was used for better understanding of this gene.

methodsA total of 75 dogs which are reared as pets with or without showing any skin lesions were examined during the study period. Skin scrapings were examined by indirect method using 10% potassium hydroxide solution under 10 × microscope. DNA samples were extracted from positive skin samples and were subjected to PCR for molecular identification.

resultsA total of 25 dogs irrespective of age, sex, breed or coat showed positive result for D. canis. The PCR revealed a single amplified product of 339 bp length which exactly matched with D. canis. The chitin synthase gene was amplified by PCR, subsequently cloned, sequenced, and compared with available data in GenBank for the particular gene of D. canis. Only one single nucleotide polymorphism (SNP) was noticed at 231 position of the chitin synthase gene sequence when compared to other isolates.

conclusionThe molecular technique confirms with the morphological identity of D. canis. This report signifies the value of peculiar tool to identify 'follicular mite' even from apparently healthy skin.

Indexed as

AcariAnimalsChitin SynthaseCloning, MolecularDNADog DiseasesDogsIndiaMite InfestationsParasitologyPolymerase Chain ReactionPolymorphism, Single NucleotideSequence Analysis, DNASkinChitin SynthaseDNAChitin synthase geneCloning and sequencingDemodex canisPhylogenetic analysis

Identifiers

PMID30645735
OpenAlexW2909337681

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.