ArticleScientific reports2019
Modernization of Golgi staining techniques for high-resolution, 3-dimensional imaging of individual neurons.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Fluorescent Labeling Methods for Brain Structure Research.Molecules (Basel, Switzerland) · 2026Review
- High-Resolution Single-Neuron Reconstruction Analysis in Golgi-Stained Brain Tissues.Cell proliferation · 2026Article
- Back to the future: improving storage of Golgi-stained mouse brain.European journal of histochemistry : EJH · 2026Article
- Golgi-Cox Staining Protocol for Medium Spiny Neurons in the Striatum of Neonatal and Early Postnatal Mouse Brain Using Cryosections.International journal of molecular sciences · 2025Article
- Investigating the effects of Ginkgo biloba leaf extract on cognitive function in Alzheimer's disease.CNS neuroscience & therapeutics · 2024Article
- Treadmill exercise prevents recognition memory impairment in VD rat model and enhancement of hippocampal structural synaptic plasticity.Brain and behavior · 2024Article
- Arabidopsis TETRASPANIN8 mediates exosome secretion and glycosyl inositol phosphoceramide sorting and trafficking.The Plant cell · 2024Article
- Reduction in the olfactory ability in agingFrontiers in aging · 2024Article
- Deletion of Small GTPase H-Ras Rescues Memory Deficits and Reduces Amyloid Plaque-Associated Dendritic Spine Loss in Transgenic Alzheimer's Mice.Molecular neurobiology · 2023Article
- Techniques to Render Dendritic Spines Visible in the Microscope.Advances in neurobiology · 2023Article
- Introduction: What Are Dendritic Spines?Advances in neurobiology · 2023Article
- ERRγ Ligand Regulates Adult Neurogenesis and Depression-like Behavior in a LRRK2-G2019S-associated Young Female Mouse Model of Parkinson's Disease.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2022Article
- Expansion-Based Clearing of Golgi-Cox-Stained Tissue for Multi-Scale Imaging.International journal of molecular sciences · 2022Article
- Three-axis classification of mouse lung mesenchymal cells reveals two populations of myofibroblasts.Development (Cambridge, England) · 2022Article
- A revisit to staining reagents for neuronal tissues.Annals of eye science · 2022Article
- A combined use of silver pretreatment and impregnation with consequent Nissl staining for cortex and striatum architectonics study.Frontiers in neuroanatomy · 2022Article
- Imaging Synaptic Density: The Next Holy Grail of Neuroscience?Frontiers in neuroscience · 2022Review
- A workflow for streamlined acquisition and correlation of serial regions of interest in array tomography.BMC biology · 2021Article
- Antidepressant-like effect of guanosine involves activation of AMPA receptor and BDNF/TrkB signaling.Purinergic signalling · 2021Article
- Argentic staining reveals changes in cerebellar tissue organisation by prenatal glucocorticoid administration in rats.Histology and histopathology · 2021Article
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9 authors.
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Abstract
Analysis of neuronal arborization and connections is a powerful tool in fundamental and clinical neuroscience. Changes in neuronal morphology are central to brain development and plasticity and are associated with numerous diseases. Golgi staining is a classical technique based on a deposition of metal precipitate in a random set of neurons. Despite their versatility, Golgi methods have limitations that largely precluded their use in advanced microscopy. We combined Golgi staining with fluorescent labeling and tissue clearing techniques in an Alzheimer's disease model. We further applied 3D electron microscopy to visualize entire Golgi-stained neurons, while preserving ultrastructural details of stained cells, optimized Golgi staining for use with block-face scanning electron microscopy, and developed an algorithm for semi-automated neuronal tracing of cells displaying complex staining patterns. Our method will find use in fundamental neuroscience and the study of neuronal morphology in disease.
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